A preliminary study of the localization of infectious laryngotracheitis virus glycoprotein E within specific peripheral blood lymphocytes

Autor:	Shahid Nazir, Richard P. G. Charlesworth, Pierre D. J. Moens, Awol M. Assen, Stephen W. Walkden-Brown, Priscilla F. Gerber
Rok vydání:	2022
Předmět:	Herpesvirus 1 Gallid General Immunology and Microbiology Food Animals Animals Pilot Projects Viral Vaccines Animal Science and Zoology Herpesviridae Infections Lymphocytes Chickens Poultry Diseases Glycoproteins
Zdroj:	Avian Pathology. 51:141-145
ISSN:	1465-3338 0307-9457
DOI:	10.1080/03079457.2021.2015062
Popis:	Infectious laryngotracheitis virus (ILTV) DNA has been detected in blood fractions, but the cell phenotype with which the virus is associated is unknown. This study investigated the presence of ILTV antigen in peripheral blood cells of six acutely ILTV-infected chickens (5 or 9 days post ocular inoculation with a virulent isolate) and three sham-inoculated chickens using immunofluorescent staining. Blood fractions were separated by Ficoll-Paque density gradient centrifugation, and smears were prepared from erythrocyte and leukocyte fractions. The smears were stained for ILTV glycoprotein E and the leukocyte markers CD4, CD8, Bu-1 (B cell), KUL01 (monocyte/macrophage), TCRγδ, and TCRαβ/Vβ
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::455626f1a86b256fc3107d7ed9f7d462 https://doi.org/10.1080/03079457.2021.2015062 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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