A novel triple repeat mutant tau transgenic model that mimics aspects of pick's disease and fronto-temporal tauopathies
| Autor: | Eliezer Masliah, Anthony Adame, Edward Rockenstein, Christina Patrick, Margarita Trejo-Morales, Michael Mante, Brian Spencer, Cassia R. Overk, Kiren Ubhi, Alejandro Bisquert |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Repetitive Sequences
Amino Acid Pathology medicine.medical_specialty Time Factors Intermediate Filaments lcsh:Medicine Hippocampus Mice Transgenic Neocortex tau Proteins Biology Hippocampal formation Progressive supranuclear palsy Mice Limbic system Pick Disease of the Brain Memory mental disorders medicine Corticobasal degeneration Animals Humans RNA Messenger lcsh:Science Multidisciplinary Behavior Animal Dentate gyrus lcsh:R medicine.disease Axons Cell biology Mitochondria Disease Models Animal Protein Transport medicine.anatomical_structure Gene Expression Regulation Tauopathies lcsh:Q Pick's disease Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 3, p e0121570 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Tauopathies are a group of disorders leading to cognitive and behavioral impairment in the aging population. While four-repeat (4R) Tau is more abundant in corticobasal degeneration, progressive supranuclear palsy, and Alzheimer's disease, three-repeat (3R) Tau is the most abundant splice, in Pick's disease. A number of transgenic models expressing wild-type and mutant forms of the 4R Tau have been developed. However, few models of three-repeat Tau are available. A transgenic mouse model expressing three-repeat Tau was developed bearing the mutations associated with familial forms of Pick's disease (L266V and G272V mutations). Two lines expressing high (Line 13) and low (Line 2) levels of the three-repeat mutant Tau were analyzed. By Western blot, using antibodies specific to three-repeat Tau, Line 13 expressed 5-times more Tau than Line 2. The Tau expressed by these mice was most abundant in the frontal-temporal cortex and limbic system and was phosphorylated at residues detected by the PHF-1, AT8, CP9 and CP13 antibodies. The higher-expressing mice displayed hyperactivity, memory deficits in the water maze and alterations in the round beam. The behavioral deficits started at 6-8 months of age and were associated with a progressive increase in the accumulation of 3R Tau. By immunocytochemistry, mice from Line 13 displayed extensive accumulation of 3R Tau in neuronal cells bodies in the pyramidal neurons of the neocortex, CA1-3 regions, and dentate gyrus of the hippocampus. Aggregates in the granular cells had a globus appearance and mimic Pick's-like inclusions. There were abundant dystrophic neurites, astrogliosis and synapto-dendritic damage in the neocortex and hippocampus of the higher expresser line. The hippocampal lesions were moderately argyrophilic and Thioflavin-S negative. By electron microscopy, discrete straight filament aggregates were detected in some neurons in the hippocampus. This model holds promise for better understanding the natural history and progression of 3R tauopathies and their relationship with mitochondrial alterations and might be suitable for therapeutical testing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|