Lipid transfer protein sensitization: reactivity profiles and clinical risk assessment in an Italian cohort
| Autor: | O. De Pità, Lorenzo Cecchi, M. Giani, Damiano Abeni, E. C. Guerra, Enrico Scala, R Asero, L. Pirrotta, Roberto Paganelli, Stephen J. Till, D. Pomponi |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Allergy Adolescent Immunology Immunoglobulin E medicine.disease_cause Risk Assessment Severity of Illness Index Cohort Studies Young Adult Mugwort Pollen Hypersensitivity Prevalence medicine Cluster Analysis Humans Immunology and Allergy Child Sensitization Aged biology Microarray analysis techniques Allergens Middle Aged medicine.disease medicine.anatomical_structure Italy Profilin Child Preschool biology.protein Female Immunization Carrier Proteins Plant lipid transfer proteins Food Hypersensitivity |
| Zdroj: | Allergy. 70:933-943 |
| ISSN: | 0105-4538 |
| DOI: | 10.1111/all.12635 |
| Popis: | Nonspecific lipid transfer proteins (nsLTPs) represent a major cause of systemic food allergic reactions in the Mediterranean area. This study investigate hierarchical patterns and cluster relationships of IgE sensitization to different nsLTPs, and the relationship to clinical allergy in a large Italian cohort.A total of 568 nsLTP-positive subjects after IgE ImmunoCAP-ISAC microarray analysis with Ara h 9, Art v 3, Cor a 8, Jug r 3, Pla a 3, Pru p 3 and Tri a 14 allergens were studied. IgE inhibition experiments were carried out with mugwort and plane tree pollen extracts.Eighty-two per cent of nsLTP-positive participants (94% if6 years old) were Pru p 3(pos) , and 71% were Jug r 3(pos) . Participants who reacted to5 nsLTPs reported a higher incidence of food-induced systemic reactions. Only Art v 3 and Pla a 3 (mugwort and plane tree nsLTPs, respectively) were associated with respiratory symptoms, and a correlation was observed between sensitization to pollen and plant food nsLTPs, particularly between Pla a 3 and tree nut/peanut nsLTPs. Co-sensitization to Par j 2 and PR-10 or profilin pan-allergens was associated with a lower prior prevalence of severe food-induced reactions. In inhibition assays, plane and mugwort pollen extracts inhibited 50-100% of IgE binding to food nsLTPs in microarrays.Testing IgE reactivity to a panel of nsLTP allergens unveils important associations between nsLTP sensitization profiles and clinical presentation and allows the identification of novel cluster patterns indicating likely cross-reactivities and highlighting potential allergens for nsLTP immunotherapy. |
| Databáze: | OpenAIRE |
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