Humoral and Cellular Responses to a Single Dose of Fendrix in Renal Transplant Recipients with Non-response to Previous Hepatitis B Vaccination
| Autor: | Marina Zaslavskaya, Melanie Fiedler, Benjamin Wilde, Monika Lindemann, Peter A. Horn, Oliver Witzke, Falko M. Heinemann, Andreas Heinold |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male Enzyme-Linked Immunospot Assay Cellular immunity Immunology Medizin Lymphocyte proliferation Antibodies Viral medicine.disease_cause Interferon-gamma Young Adult 03 medical and health sciences 0302 clinical medicine Immunity Immune Tolerance medicine Humans Hepatitis B Vaccines 030212 general & internal medicine Hepatitis B Antibodies Cells Cultured Aged Cell Proliferation Hepatitis B virus Immunity Cellular biology business.industry ELISPOT Vaccination virus diseases General Medicine Hepatitis B medicine.disease Kidney Transplantation Virology digestive system diseases Immunity Humoral biology.protein Female 030211 gastroenterology & hepatology Antibody business |
| Zdroj: | Scandinavian Journal of Immunology. 85:51-57 |
| ISSN: | 0300-9475 |
| Popis: | Approximately 70% of kidney transplant recipients are non-responders to conventional hepatitis B virus (HBV) vaccines. We examined whether Fendrix™, an HBV vaccine containing 3-O-desacyl-4'-monophosphoryl lipid A (MPL) as adjuvant, could induce HBV immunity in these patients and compared their vaccination efficacy with healthy controls tested previously by the same assays. We selected 35 kidney transplant recipients who had been vaccinated at least thrice against HBV but had never displayed anti-HBs antibodies. We re-assessed their anti-HBs antibody titres and further determined cellular HBV immunity by proliferation assay and interferon (IFN)-γ ELISpot. Seventeen recipients did neither display humoral nor cellular immunity and could be tested prior to and at month 1 after vaccination. Of note, HLA antigens associated with non-response to HBV vaccination (HLA-DRB1*03 and HLA-DQB1*02) were over-represented in these 17 recipients. At month 1 after a single vaccination with Fendrix™, we observed a significant increase in anti-HBs antibodies (P = 0.02). In seven of 17 recipients, we detected anti-HBs antibodies ≥10 IU/l (10-264), in four HBV-specific lymphocyte proliferation (stimulation index of 2.6-8.7) and in one specific IFN-γ responses (12 spots increment). The vaccination response to Fendrix™ was significantly higher (P = 0.035) than the response to HBVaxPro™ in young healthy controls. In summary, the results show that a single vaccination with Fendrix™ could already induce HBV-specific humoral and/or cellular responses in ten of 17 kidney transplant patients. Thus, Fendrix™ appears as an efficient vaccine in this patient cohort. |
| Databáze: | OpenAIRE |
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