Sequence-Dependent Basepair Opening in DNA Double Helix
| Autor: | Andrew S Krueger, Ekaterina Protozanova, Maxim D. Frank-Kamenetskii |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Base Sequence
Base pair Hydrogen bond Chemistry Entropy Stacking Temperature Biophysics Thermal fluctuations Nuclear magnetic resonance spectroscopy DNA Biophysical Theory and Modeling Nucleobase chemistry.chemical_compound Kinetics Nuclear magnetic resonance Chemical physics Pairing Nucleic Acid Conformation Base Pairing |
| Zdroj: | Biophysical Journal. 90(9):3091-3099 |
| ISSN: | 0006-3495 |
| DOI: | 10.1529/biophysj.105.078774 |
| Popis: | Preservation of genetic information in DNA relies on shielding the nucleobases from damage within the double helix. Thermal fluctuations lead to infrequent events of the Watson-Crick basepair opening, or DNA “breathing”, thus making normally buried groups available for modification and interaction with proteins. Fluctuational basepair opening implies the disruption of hydrogen bonds between the complementary bases and flipping of the base out of the helical stack. Prediction of sequence-dependent basepair opening probabilities in DNA is based on separation of the two major contributions to the stability of the double helix: lateral pairing between the complementary bases and stacking of the pairs along the helical axis. The partition function calculates the basepair opening probability at every position based on the loss of two stacking interactions and one basepairing. Our model also includes a term accounting for the unfavorable positioning of the exposed base, which proceeds through a formation of a highly constrained small loop, or a ring. Quantitatively, the ring factor is found as an adjustable parameter from the comparison of the theoretical basepair opening probabilities and the experimental data on short DNA duplexes measured by NMR spectroscopy. We find that these thermodynamic parameters suggest nonobvious sequence dependent basepair opening probabilities. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|