Medium-high resolution electrochemical genotyping of HLA-DQ2/DQ8 for detection of predisposition to coeliac disease
| Autor: | Jukka Partanen, Hamdi Joda, Daniel Latta, Ciara K. O'Sullivan, Noora Alakulppi, Valerio Beni, Kristina Lind, Ioanis Katakis, Linda Strömbom, Julian Höth |
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| Rok vydání: | 2014 |
| Předmět: |
Genotype
Human leukocyte antigen Biology 01 natural sciences Biochemistry Coeliac disease Analytical Chemistry 03 medical and health sciences 0302 clinical medicine HLA-DQ Antigens medicine Humans Multiplex Allele Genotyping Alleles chemistry.chemical_classification 010401 analytical chemistry HLA-DQ2 nutritional and metabolic diseases Chromosome Electrochemical Techniques Microarray Analysis medicine.disease Molecular biology Gluten 0104 chemical sciences Celiac Disease chemistry Disease Susceptibility 030215 immunology |
| Zdroj: | Analytical and Bioanalytical Chemistry. 406:2757-2769 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-014-7650-x |
| Popis: | Coeliac disease is a small intestinal disorder, induced by ingestion of gluten in genetically predisposed individuals. Coeliac disease has been strongly linked to human leukocyte antigens (HLA) located on chromosome 6, with almost 100 % of coeliac disease sufferers carrying either a HLA-DQ2 or HLA-DQ8 heterodimer, with the majority carrying HLA-DQ2 encoded by the DQA1*05:01/05:05, DQB1*02:01/02:02 alleles, whereas the remaining carry the HLA-DQ8 encoded by the DQA1*03:01, DQB1*03:02 alleles. In this work, we present the development of a multiplex electrochemical genosensor array of 36 electrodes, housed within a dedicated microfluidic platform and using a total of 10 sequence-specific probes for rapid medium-high resolution HLA-DQ2/DQ8 genotyping. An evaluation of the selectivity of the designed probes was carried out with the target sequences and 44 potentially interfering alleles, including single base mismatch differentiations; good selectivity was demonstrated. The performance of the electrochemical genosensor array was validated, analyzing real human samples for the presence of HLA-DQ2/DQ8 alleles, and compared with those obtained using laboratory-based HLA typing, and an excellent correlation was obtained. |
| Databáze: | OpenAIRE |
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