Subacute treatment of carprofen facilitate splenocardiac resolution deficit in cardiac injury
| Autor: | Vasundhara Kain, Griffin M. Wright, Ganesh V. Halade, Jeevan Kumar Jadapalli |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Phagocytosis Heart Ventricles Immunology Carbazoles Myocardial Infarction Inflammation Spleen Apoptosis 030204 cardiovascular system & hematology Biology Neutrophil Activation Article Proinflammatory cytokine 03 medical and health sciences Mice 0302 clinical medicine medicine Leukocytes Immunology and Allergy Animals Carprofen Myocardial infarction Heart Failure Anti-Inflammatory Agents Non-Steroidal Membrane Proteins Cell Biology Macrophage Activation medicine.disease Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Coronary occlusion Cyclooxygenase 2 Heart failure Cyclooxygenase 1 Prostaglandins Eicosanoids medicine.symptom Inflammation Mediators medicine.drug |
| Zdroj: | J Leukoc Biol |
| ISSN: | 1938-3673 |
| Popis: | Inflammation-limiting nonsteroidal pain relievers magnify myocardial infarction (MI) incidences and increase re-admission events in heart failure (HF) patients. However, the molecular and cellular mechanism of this provocative adverse effect is unclear. Our goal was to determine whether carprofen (CAP) impedes splenic leukocyte-directed acute inflammation-resolving response in cardiac injury. After subacute CAP treatment, mice were subjected to permanent coronary ligation maintaining MI- and naïve-controls. Spleen and left ventricle (LV) leukocytes were quantitated using flow cytometry pre- and 24 h post-MI. The inflammation resolution mediators were quantified using mass spectrometry while splenocardiac apoptosis and leukocyte phagocytosis were measured by immunofluorescence and ImageStream, respectively. Subacute CAP treatment promoted strain and cardiac dysfunction before MI and coronary occlusion showed signs of acute HF in CAP and MI-controls. Subacute CAP-injected mice had pre-activated splenic neutrophils, an over activated “don't eat me” signal (CD47) with reduced total Mϕs (F4/80+) and reparative Mϕs (F4/80/Ly6Clo/CD206) compared with control in LV and spleen. Post-MI, CAP pre-activated neutrophils (Ly6G+) were intensified and reduced reparative neutrophils (Ly6G+/CD206+) and Mϕs (F4/80/Ly6Clo) in LV was indicative of non-resolving inflammation compared with MI-control. Subacute CAP treatment deferred neutrophil phagocytosis functions in the spleen and LV and was more evident post-MI compared with MI-control. CAP pre-activated splenic neutrophils that tailored the Mϕ phagocytosis thereby increased splenocardiac leukocyte death. CAP over amplified COX-1 and COX-2 compared with MI-control and failed to limit prostaglandins and thromboxane in post-MI setting. Further, CAP reduced cardiac-protective epoxyeicosatrienoic acids and over amplified pyrogenic inflammatory cytokines and reduced reparative cytokines, thereby non-resolving inflammation. Inflammation-limiting pain relievers magnify myocardial infarction incidences, and therefore redefined the mechanism that subacute carprofen treatment in mice pre-activates splenic neutrophils with signs of nonresolving inflammation. |
| Databáze: | OpenAIRE |
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