Investigations withO-linked protein glycosylations by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry
| Autor: | Kristina L. Toups, Taufika Islam Williams, Diana A. Saggese, Jennifer L. Frahm, David C. Muddiman, Hyun Joo An, Carlito B. Lebrilla, Bensheng Li |
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| Rok vydání: | 2008 |
| Předmět: |
Glycan
Angiotensins Glycosylation Swine Analytical chemistry Oligosaccharides Mass spectrometry Ion cyclotron resonance spectrometry Tandem mass spectrometry Article Fourier transform ion cyclotron resonance chemistry.chemical_compound Tandem Mass Spectrometry Spectroscopy Fourier Transform Infrared Animals Sample preparation Spectroscopy Chromatography biology Chemistry Mucins Reproducibility of Results Matrix-assisted laser desorption/ionization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein |
| Zdroj: | Journal of Mass Spectrometry. 43:1215-1223 |
| ISSN: | 1096-9888 1076-5174 |
| DOI: | 10.1002/jms.1398 |
| Popis: | Posttranslational modifications such as glycosylation can play a fundamental role in signaling pathways that transform an ordinary cell into a malignant one. The development of a protocol to detect these changes in the preliminary stages of disease can lead to a sensitive and specific diagnostic for the early detection of malignancies such as ovarian cancer in which differential glycan patterns are linked to etiology and progression. Small variations in instrument parameters and sample preparation techniques are known to have significant influence on the outcome of an experiment. For an experiment to be effective and reproducible, these parameters must be optimized for the analyte(s) under study. We present a detailed examination of sample preparation and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FT-ICR-MS) analysis of O-linked glycans globally cleaved from mucin glycoproteins. Experiments with stable isotope-labeled biomolecules allowed for the establishment of appropriate acquisition times and excitation voltages for MALDI-FT-ICR-MS of oligosaccharides. Quadrupole ion guide optimization studies with mucin glycans identified conditions for the comprehensive analysis of the entire mass range of O-linked carbohydrates in this glycoprotein. Separately optimized experimental parameters were integrated in a method that allowed for the effective study of O-linked glycans. |
| Databáze: | OpenAIRE |
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