Determining the maximal storage time of centrifuged citrated samples for performing add-on routine coagulation tests
Autor: | Yvonne M. C. Henskens, E.J.S. Denessen, M.L.J. Jeurissen, R.M.T.A. Pereboom, Paul W.M. Verhezen |
---|---|
Přispěvatelé: | Faculteit FHML Centraal, RS: Carim - B01 Blood proteins & engineering, MUMC+: DA CDL Analytisch cluster 1K (9), MUMC+: DA CDL Algemeen (9), RS: NUTRIM - R2 - Liver and digestive health |
Rok vydání: | 2020 |
Předmět: |
Routine coagulation tests
INR PREANALYTICAL VARIABLES PARTIAL THROMBOPLASTIN TIME Thrombin Time 030204 cardiovascular system & hematology Thrombin time Fibrinogen 03 medical and health sciences 0302 clinical medicine WHOLE-BLOOD medicine Coagulation testing Humans Coagulation (water treatment) Citrates Whole blood Prothrombin time Chromatography STABILITY medicine.diagnostic_test Chemistry Pre-analytical variables Hematology PROTHROMBIN TIME SPECIMENS Cell Pellet ROOM-TEMPERATURE 030220 oncology & carcinogenesis Blood Coagulation Tests LABORATORY CONTROL ANTICOAGULANT-THERAPY Maximal storage times medicine.drug Partial thromboplastin time |
Zdroj: | Thrombosis Research, 196, 54-62. Elsevier Science |
ISSN: | 0049-3848 |
Popis: | Background Several routine coagulation tests have been developed to give insight in the coagulation pathway. The laboratory diagnostical process consists of 3 phases, the pre-analytical, analytical and post-analytical phase; however, the pre-analytical phase is most sensitive to errors. The amount of time blood is stored, can affect the measurements of these coagulation tests and result in an incorrect conclusion. Therefore, we performed experiments to determine the maximal storage time, centrifuged blood samples can be reliably measured. Methods Citrated whole blood from hospital patients, who were tested for routine coagulation, was collected in 2.7 mL citrate tubes. These whole blood samples were centrifuged and the plasma was stored on top of the cell pellet at room temperature. After 2 h, 4 h, 6 h, 12 h and 24 h, the prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), fibrinogen concentration, antithrombin activity, D-dimer concentration and thrombin time were measured using Sysmex CS2100 coagulation analysers. Results and conclusion Analytical evaluation of routine coagulation tests resulted in various significant differences and large variations between the various time intervals. Our results indicated that the PT and INR can be measured up till 24 h of storage. Centrifuged blood for measuring the fibrinogen concentration, antithrombin activity, D-dimer concentration and thrombin time can be stored up to 4 h, while 2 h of storage might already be too long for obtaining reliable aPTT measurements. |
Databáze: | OpenAIRE |
Externí odkaz: |