Molecular characterization of OP354-like P[8] (P[8]b subtype) human rotaviruses a species isolated in Japan
| Autor: | Kouji Kida, Tomohisa Kanadani, Mitsutaka Kuzuya, Ritsushi Fujii, Toshio Kishimoto, Masako Hamano, Keiko Nishimura |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Rotavirus Adolescent Genes Viral Genotype viruses Molecular Sequence Data Reassortment Biology Southeast asian Viral Proteins Japan Virology Cluster Analysis Humans Child Gene Phylogeny Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Infant Newborn Nucleic acid sequence Infant virus diseases Sequence Analysis DNA Molecular analysis Reverse transcription polymerase chain reaction Infectious Diseases Child Preschool Vp4 gene RNA Viral Female |
| Zdroj: | Journal of Medical Virology. 84:697-704 |
| ISSN: | 0146-6615 |
| DOI: | 10.1002/jmv.23224 |
| Popis: | OP354-like P[8] (P[8]b subtype) species A rotaviruses (RVAs) were isolated first time in Japan during a RVA survey in Okayama Prefecture between 2006 and 2009. Two of 236 RVA-positive samples were identified as G1P[8]b by reverse transcription polymerase chain reaction. P[8]b strains (RVA/human-wt/JPN/OH1998/2008/G1P[8]b and RVA/human-wt/JPN/OH2024/2008/G1P[8]b) were isolated only in May, 2008 and both patients infected with P[8]b viruses lived in the same city, suggesting that the prevalence of P[8]b RVAs is limited considerably in Okayama Prefecture. Molecular analysis of four genes (VP4, VP6, VP7, and NSP4 genes) of Japanese P[8]b strains revealed that the VP4 genes of these strains were related closely to those of Southeast Asian and Indian P[8]b strains. In contrast, the VP6, VP7, and NSP4 genes of Japanese P[8]b strains were highly homologous to G1P[8]a strains prevalent in the same area. These results suggest that the Japanese P[8]b strain may be a result of reassortment events between Japanese G1P[8]a viruses and unidentified Asian viruses possessing the P[8]b VP4 gene. J. Med. Virol. 84:697–704, 2012. © 2011 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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