Preconcentration and Detection of Gefitinib Anti-Cancer Drug Traces from Water and Human Plasma Samples by Means of Magnetic Nanoparticles
| Autor: | Borg, Hardeer, Zámbó, Dániel, Elmansi, Heba, Hashem, Heba M., Nasr, Jenny Jehan, Walash, Mohammed I., Bigal, Nadja C., Belal, Fathalla |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Chemistry
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie lcsh:QD1-999 human plasma Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften Biologie ddc:570 ddc:540 gefitinib iron oxide nanoparticles liquid chromatography magnetic solid-phase extraction Article |
| Zdroj: | Nanomaterials Nanomaterials, Vol 10, Iss 1196, p 1196 (2020) Volume 10 Issue 6 Nanomaterials 10 (2020), Nr. 6 |
| DOI: | 10.15488/10927 |
| Popis: | Along of widespread application of anti-cancer drug Gefitinib (GEF), it appears in human body fluids as well as clinical wastewater. Consequently, a reliable and easy-to-adapt detection technique is of essential importance to quantify the drug in different media. The extraction and quantitative detection of anti-cancer drug Gefinitib (GEF) is demonstrated based on a straightforward and efficient magnetic nanoparticle-assisted preconcentration route from water and human plasma samples. Iron oxide magnetic nanoparticles (Fe3O4) have been prepared with an average particle size of 15 nm and utilized as extractible adsorbents for the magnetic solid-phase extraction (MSPE) of GEF in aqueous media. The method is based on MSPE and preconcentration of GEF followed by High-Performance Liquid Chromatography-Ultraviolet Detection (HPLC-UV). The yield of GEF extraction under the optimum MSPE conditions were 94% and 87% for water and plasma samples, respectively. The chromatographic separation was carried out isocratically at 25 ° C on a Phenomenex C8 reversed phase column (150 mm × 4.6 mm, with 5 µ m particle size). The proposed method was linear over concentration ranges of 15.0&ndash 300.0 and 80.0&ndash 600.0 ng/mL for water and plasma samples with limits of detection of 4.6 and 25.0 ng/mL in a respective order. Relative standard deviations (%RSD) for intra-day and inter-day were 0.75 and 0.94 for water samples and 1.26 and 1.70 for plasma samples, respectively. Using the magnetic nanoparticles (MNPs) as loaded drug-extractors made the detection of the anti-cancer drug environmentally friendly and simple and has great potential to be used for different drug-containing systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|