The RabGAPs EPI64A and EPI64B regulate the apical structure of epithelial cells †
| Autor: | Anthony Bretscher, Matthew R Miller, Riasat Zaman, Andrew J Lombardo, David J. McDermitt, Cécile Sauvanet |
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| Rok vydání: | 2022 |
| Předmět: |
Scaffold protein
Cell Biology Basal (phylogenetics) Ezrin Cell Line Tumor medicine Humans CRISPR Phosphorylation Molecular Biology Adaptor Proteins Signal Transducing Binding Sites Microvilli GTPase-Activating Proteins Cell Polarity Epithelial Cells Cell Biology Phosphoproteins Epithelium Cell biology Cytoskeletal Proteins medicine.anatomical_structure Severe phenotype Caco-2 Cells Function (biology) Protein Binding |
| Zdroj: | Molecular Biology of the Cell. 33 |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e21-05-0268 |
| Popis: | Here we report on the related TBC/RabGAPs EPI64A and EPI64B and show that they function to organize the apical aspect of epithelial cells. EPI64A binds the scaffolding protein EBP50/NHERF1, which itself binds active ezrin in epithelial cell microvilli. Epithelial cells additionally express EPI64B that also localizes to microvilli. However, EPI64B does not bind EBP50 and both proteins are shown to have a microvillar localization domain that spans the RabGAP domains. CRISPR/Cas9 was used to inactivate expression of each protein individually or both in Jeg-3 and Caco2 cells. In Jeg-3 cells, loss of EPI64B resulted in a reduction of apical microvilli, and a further reduction was seen in the double knockout, mostly likely due to misregulation of Rab8 and Rab35. In addition, apical junctions were partially disrupted in cells lacking EPI64A, and accentuated in the double knock out. In Caco2 loss of EPI64B resulted in wavy junctions, whereas loss of both EPI64A and EPI64B had a severe phenotype often resulting in cells with a stellate apical morphology. In the knockout cells, the basal region of the cell remained unchanged, so EPI64A and EPI64B specifically localize to and regulate the morphology of the apical domain of polarized epithelial cells. |
| Databáze: | OpenAIRE |
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