In vivo transcriptional products of the chloroplast DNA of Euglena gracilis

Autor:	James R. Y. Rawson, Kim P. Dix
Rok vydání:	1983
Předmět:	Euglena gracilis ved/biology ved/biology.organism_classification_rank.species food and beverages RNA General Medicine Biology Genome Molecular biology Chloroplast chemistry.chemical_compound Restriction enzyme Plasmid chemistry Chloroplast DNA Genetics DNA
Zdroj:	Current Genetics. 7:265-272
ISSN:	1432-0983 0172-8083
DOI:	10.1007/bf00376071
Popis:	Cloned chloroplast restriction endonuclease DNA fragments were used as hybridization probes to identify the in vivo transcriptional products of the chloroplast genome from the alga Euglena gracilis. Total cellular RNA was size fractionated by electrophoresis in denaturing gels and transferred to nitrocellulose paper. Individual plasmids containing specific chloroplast DNA fragments were radioactively labeled in vitro and hybridized to the immobilized RNA. The stable RNAs in the chloroplast were identified on the basis of their size and their origin on the chloroplast genome. Several transcripts were shown to be developmentally expressed. Some transcripts showed a possible precursor-product relationship. The rDNA was shown to be transcribed as a large transcript and then processed to the, mature rRNAs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44b47998c0e3f218b76de6c875368d5f https://doi.org/10.1007/bf00376071 Zobrazit plný text záznamu Full text from SpringerLink