Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages
Autor: | Hyo-Jin An, Divina C. Cominguez, Zhiyun Zhang, Se-Yun Cheon, Yea-Jin Park, Dong-Sung Lee, Lee Sang Woo |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Lipopolysaccharides
Male MAPK/ERK pathway Article Subject Lipopolysaccharide NF-E2-Related Factor 2 medicine.drug_class Immunology Anti-Inflammatory Agents Tetrazolium Salts Inflammation Asteraceae Pharmacology medicine.disease_cause Antioxidants Dinoprostone Anti-inflammatory Proinflammatory cytokine Nitric oxide Mice chemistry.chemical_compound Sepsis Pathology medicine Animals RB1-214 Extracellular Signal-Regulated MAP Kinases chemistry.chemical_classification Reactive oxygen species Plant Extracts Chemistry Macrophages Methanol Membrane Proteins Cell Biology Mice Inbred C57BL Oxidative Stress Thiazoles RAW 264.7 Cells medicine.symptom Reactive Oxygen Species Heme Oxygenase-1 Oxidative stress Research Article |
Zdroj: | Mediators of Inflammation Mediators of Inflammation, Vol 2020 (2020) |
ISSN: | 1466-1861 0962-9351 |
Popis: | A hypernomic reaction or an abnormal inflammatory process could cause a series of diseases, such as cardiovascular disease, neurodegeneration, and cancer. Additionally, oxidative stress has been identified to induce severe tissue injury and inflammation. Carpesium cernuum L. (C. cernuum) is a Chinese folk medicine used for its anti-inflammatory, analgesic, and detoxifying properties. However, the underlying molecular mechanism of C. cernuum in inflammatory and oxidative stress conditions remains largely unknown. The aim of this study was to examine the effects of a methanolic extract of C. cernuum (CLME) on lipopolysaccharide- (LPS-) induced RAW 264.7 mouse macrophages and a sepsis mouse model. The data presented in this study indicated that CLME inhibited LPS-induced production of proinflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 cells. CLME treatment also reduced reactive oxygen species (ROS) generation and enhanced the expression of heme oxygenase-1 (HO-1) protein in a dose-dependent manner in the LPS-stimulated RAW 264.7 cells. Moreover, CLME treatment abolished the nuclear translocation of nuclear factor-κB (NF-κB), enhanced the activation of nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), and reduced the expression of extracellular signal-related kinase (ERK) and ERK kinase (MEK) phosphorylation in LPS-stimulated RAW 264.7 cells. These outcomes implied that CLME could be a potential antioxidant and anti-inflammatory agent. |
Databáze: | OpenAIRE |
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