Characterization of a new phosphonocerebroside, N-methyl-2-aminoethylphosphonylglucosylceramide, from the antarctic krill, Euphausia superba
| Autor: | Saki Itonori, Taro Hori, Kazuo Kamemura, Osamu Itasaka, Mutsumi Sugita, Naohisa Sonku, Kenji Narushima |
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| Rok vydání: | 1991 |
| Předmět: |
Chromatography
Gas Magnetic Resonance Spectroscopy Spectrophotometry Infrared Euphausia Ion chromatography Biophysics Glucosylceramides Biochemistry Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Hydrolysis Endocrinology Column chromatography Cerebrosides Crustacea Animals Silicic acid Phosphorylation Chromatography biology biology.organism_classification chemistry Antarctic krill Euphausiacea Gas chromatography Chromatography Thin Layer Chromatography Liquid |
| Zdroj: | Biochimica et biophysica acta. 1081(3) |
| ISSN: | 0006-3002 |
| Popis: | A novel phosphonglycosphingolipid was purified from the whole tissue of the antarctic krill, Euphausia superba by successive column chromatography on DEAE- and QAE-Sephadex and silicic acid (Iatrobeads). The structure was elucidated by means of IR, FAB-MS, 1H-NMR, GC and GC-MS analyses of the water-soluble products after complete and partial acid hydrolysis, and methylation analysis of a product of hydrogen fluoride degradation; it was identified to be a phosphonocerebroside, 6'-O-(N-methyl-2-aminoethylphosphonyl)Glcp beta 1----1ceramide. The ceramide moiety was composed of tetradecasphingenine and octadecasphingatriene as the main sphingoids, and monounsaturated C22- and C24-acids and their 2-hydroxy homologues as the major fatty acids. |
| Databáze: | OpenAIRE |
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