Normalizing polydiacetylene colorimetric assays of vesicle binding across lipid systems
| Autor: | Alyssa R. Stonebraker, Maryssa Beasley, Justin Legleiter |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Amyloid beta-Peptides Chemistry High-throughput screening Vesicle Phosphatidylethanolamines education 010401 analytical chemistry Lipid Bilayers Biophysics Cell Biology 01 natural sciences Biochemistry Polyacetylene Polymer 0104 chemical sciences 03 medical and health sciences Membrane β amyloid Phosphatidylcholines Colorimetry Lipid vesicle Molecular Biology 030304 developmental biology |
| Zdroj: | Analytical biochemistry. 609 |
| ISSN: | 1096-0309 |
| Popis: | Mixed polydiacetylene (PDA) lipid vesicles mimic cell membranes and exhibit a colorimetric response induced by mechanical stress, which can be used to determine the affinity of proteins or molecules for lipid membranes. Due to a simple spectroscopic readout, PDA assays are amenable to high-throughput screens; however, these assays exhibit batch-to-batch variability. Sensitivity of the assay is also influenced by physicochemical properties associated with different lipids. Here, a method of normalizing PDA assays to reduce variability and enable direct comparison across lipid systems is described. |
| Databáze: | OpenAIRE |
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