mRNA of bovine tissue inhibitor of metalloproteinase: sequence and expression in bovine ovarian tissue
| Autor: | R. M. Luck, S. Wagner, J. Freudenstein, R. Einspanier, Karl Heinz Scheit |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
endocrine system
Molecular Sequence Data Restriction Mapping Biophysics Biology Matrix metalloproteinase Biochemistry Gene expression Animals Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Peptide sequence Glycoproteins Metalloproteinase Messenger RNA Granulosa Cells Base Sequence cDNA library Ovary Tissue Inhibitor of Metalloproteinases Cell Biology Tissue inhibitor of metalloproteinase Blotting Northern Molecular biology Theca Cattle Female |
| Zdroj: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| Popis: | A cDNA library derived from poly(A+)RNA of bovine ovary was screened with a PCR fragment comprising the coding region of human tissue inhibitor of metalloproteinase inhibitor (TIMP). From a number of positive clones, pBGR19, containing a 747 bp insert, was identified and sequenced. The derived amino acid sequence represents that of the precursor of bovine TIMP. Northern analysis reveals a TIMP specific mRNA of 800 bp. Southern analysis indicates that one gene appears to specifiy bovine TIMP. TIMP mRNA is only weakly expressed in follicular granulosa- and theca cells, whereas luteinization of the follicle is associated with an increase of expression. Expression varies with the stage of the luteal phase; it was highest in stages I and III, but low in stages II and IV of the oestrous cycle. |
| Databáze: | OpenAIRE |
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