mTORC1 controls long-term memory retrieval
| Autor: | Cynthia Katche, Jorge H. Medina, Magdalena Pereyra, Ana Belén de Landeta |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male CIENCIAS MÉDICAS Y DE LA SALUD Memory Long-Term Neurociencias Regulator Short-term memory lcsh:Medicine mTORC1 Biology Mechanistic Target of Rapamycin Complex 1 Hippocampus 03 medical and health sciences 0302 clinical medicine Retrosplenial cortex Animals Rats Wistar lcsh:Science PI3K/AKT/mTOR pathway Sirolimus INHIBITORY AVOIDANCE Multidisciplinary Long-term memory MTOR lcsh:R purl.org/becyt/ford/3.1 [https] Impaired memory MEMORY RETRIEVAL Medicina Básica 030104 developmental biology Memory Short-Term Synaptic plasticity purl.org/becyt/ford/3 [https] lcsh:Q biological phenomena cell phenomena and immunity Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 2045-2322 |
| Popis: | Understanding how stored information emerges is a main question in the neurobiology of memory that is now increasingly gaining attention. However, molecular events underlying this memory stage, including involvement of protein synthesis, are not well defined. Mammalian target of rapamycin complex 1 (mTORC1), a central regulator of protein synthesis, has been implicated in synaptic plasticity and is required for memory formation. Using inhibitory avoidance (IA), we evaluated the role of mTORC1 in memory retrieval. Infusion of a selective mTORC1 inhibitor, rapamycin, into the dorsal hippocampus 15 or 40 min but not 3 h before testing at 24 h reversibly disrupted memory expression even in animals that had already expressed IA memory. Emetine, a general protein synthesis inhibitor, provoked a similar impairment. mTORC1 inhibition did not interfere with short-term memory retrieval. When infused before test at 7 or 14 but not at 28 days after training, rapamycin impaired memory expression. mTORC1 blockade in retrosplenial cortex, another structure required for IA memory, also impaired memory retention. In addition, pretest intrahippocampal rapamycin infusion impaired object location memory retrieval. Our results support the idea that ongoing protein synthesis mediated by activation of mTORC1 pathway is necessary for long but not for short term memory. Fil: Pereyra, Magdalena. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Katche, Cynthia Lorena. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: de Landeta, Ana Belén. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Medina, Jorge Horacio. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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