Mutation analysis of thep51 gene and correlation betweenp53,p73, andp51 expressions in prostatic carcinoma
| Autor: | Hiroyuki Takahashi, Taizo Shiraishi, Hiroshi Hano, Hiroyoshi Suzuki, Kazuo Fukutome, Shuntaro Ikawa, Masakuni Furusato, Masatoshi Watanabe, Haruo Ito |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Urology DNA Mutational Analysis Biology medicine.disease_cause Polymerase Chain Reaction Loss of heterozygosity Prostate Tumor Cells Cultured Carcinoma medicine Humans Genes Tumor Suppressor Gene Alleles Polymorphism Single-Stranded Conformational Aged Electrophoresis Agar Gel Mutation Tumor Suppressor Proteins Nuclear Proteins Prostatic Neoplasms Tumor Protein p73 DNA Neoplasm Sequence Analysis DNA Middle Aged Genes p53 Phosphoproteins medicine.disease DNA-Binding Proteins Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology Trans-Activators Cancer research Mutation testing Adenocarcinoma Carcinogenesis Transcription Factors |
| Zdroj: | The Prostate. 47:85-90 |
| ISSN: | 1097-0045 0270-4137 |
| DOI: | 10.1002/pros.1050 |
| Popis: | BACKGROUND p73 and p51 are genes possessing amino-acid similarities to p53. We previously found no mutation in p73 in prostatic carcinoma, but did find abnormal expression of the gene. Involvement of these genes in prostatic carcinogenesis is still poorly understood. METHODS Mutation analysis of the p51 gene and allelotyping of 3q28, on which p51 lies, were performed. Expression of p53, p73, and p51 was examined using reverse transcription–polymerase chain reaction, and expression levels were compared. RESULTS No mutation in p51 was found (0/55 cases). Loss of heterozygosity at 3q28 was detected in 6 of 28 cases (21.8%). By expression analysis we found that in p53, 4 of 38 cases (10.5%) showed downregulation. No cases showed upregulation of p53. In contrast, p73 and p51 were downregulated in 42.1 and 39.5% of cases, respectively, and upregulated in 31.5 and 34.2% of cases, respectively. Expression levels of p51 corresponded with those of p73 in 25 of 38 cases (65.8%). CONCLUSIONS Somatic mutations in p73 and p51 are not important in prostatic carcinogenesis. These genes may be associated with tumors by expression levels and may have roles in addition to tumor suppresson. Prostate 47:85–90, 2001. © 2001 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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