Strategies for effective development of ultra-sensitive LC–MS/MS assays: application to a novel STING agonist
| Autor: | Kasie Fang, Hermes Licea-Perez |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Agonist
Analyte medicine.drug_class Clinical Biochemistry 030226 pharmacology & pharmacy 01 natural sciences Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Pharmacokinetics Tandem Mass Spectrometry Lc ms ms medicine Humans General Pharmacology Toxicology and Pharmaceutics Ultra sensitive Nonspecific binding Chromatography Chemistry 010401 analytical chemistry General Medicine 0104 chemical sciences Medical Laboratory Technology Sting Biological Assay Chromatography Liquid |
| Zdroj: | Bioanalysis. 12:467-484 |
| ISSN: | 1757-6199 1757-6180 |
| Popis: | Background: The continual need for the development and validation of ultra-sensitive (low pg/ml) LC–MS/MS assays in the pharmaceutical industry is largely driven by the ultra-low analyte exposure or very low sample volume. Methodology: Strategies and systematic approaches for sensitivity enhancement are provided which cover all aspects of a LC–MS/MS bioanalysis. A case study where such strategies were applied for the validation of a 5.0 pg/ml assay for a STING agonist is discussed. Conclusion: Analytical protocols were developed to extract analytes from large volume of plasma samples (600 and 400 μl) with high throughput. The guidance provided in this publication can serve as a resource to influence LC–MS/MS method development activities. |
| Databáze: | OpenAIRE |
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