Overexpression of the Wilms' tumor geneWT1 inde novo lung cancers
| Autor: | Seiji Hayashi, Eigo Takahashi, Akihiro Tsuboi, Hiroyasu Ogawa, Shinichiro Miyoshi, Momotaro Asada, Katsuyuki Aozasa, Hajime Maeda, Haruo Sugiyama, Ichiro Kawase, Masaki Murakami, Mari Motomura, Shin-ichi Nakatsuka, Keisuke Kanato, Kazuhiro Ikegame, Yoko Nakano, Masayuki Yao, Yusuke Oji, Eui Ho Kim, Hiroya Tamaki, Naoki Hosen, Hirohisa Hirabayashi, Yoshihiro Oka, Manabu Kawakami, Yasushi Shintani, Tatsuya Fujioka |
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| Rok vydání: | 2002 |
| Předmět: |
Male
congenital hereditary and neonatal diseases and abnormalities Cancer Research Lung Neoplasms Tumor suppressor gene RNA Splicing Biology urologic and male genital diseases medicine.disease_cause Small-cell carcinoma Carcinoma Non-Small-Cell Lung medicine Humans Carcinoma Small Cell WT1 Proteins Lung cancer Gene Aged Neoplasm Staging Aged 80 and over Mutation urogenital system fungi Wilms' tumor Middle Aged medicine.disease female genital diseases and pregnancy complications Oncology Cancer research Immunohistochemistry Female Carcinogenesis |
| Zdroj: | International Journal of Cancer. 100:297-303 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.10476 |
| Popis: | Expression of the Wilms' tumor gene WT1 in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WT1 gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WT1 protein with an anti-WT1 antibody. Overexpression of the WT1 gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WT1 gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the nonmutated, wild-type WT1 gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WT1 gene and its products. |
| Databáze: | OpenAIRE |
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