Timing of exposure to gonadotropins has differential effects on the conceptus: evidence from a mouse model†
| Autor: | Marisa S. Bartolomei, Teri Ord, Chantae Sullivan-Pyke, Eric A. Rhon-Calderon, Christos Coutifaris, Monica Mainigi, Sneha Mani |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Litter Size Pregnancy Rate Gonadotropins Equine medicine.medical_treatment Placenta Mice Transgenic Superovulation Biology Chorionic Gonadotropin Drug Administration Schedule Andrology 03 medical and health sciences Mice 0302 clinical medicine Fetus Pregnancy medicine Conceptus Animals Sex Ratio reproductive and urinary physiology 030219 obstetrics & reproductive medicine In vitro fertilisation Embryogenesis Placentation Embryo Cell Biology General Medicine Organ Size DNA Methylation medicine.disease Embryo Transfer 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Fetal Weight embryonic structures Tissue and Organ Harvesting Female Research Article |
| Zdroj: | Biol Reprod |
| ISSN: | 1529-7268 |
| Popis: | Superovulation with gonadotropins alters the hormonal milieu during early embryo development and placentation, and may be responsible for fetal and placental changes observed after in vitro fertilization (IVF). We hypothesized that superovulation has differential effects depending on timing of exposure. To test our hypothesis, we isolated the effect of superovulation on pre- and peri-implantation mouse embryos. Blastocysts were obtained from either natural mating or following superovulation and mating, and were transferred into naturally mated or superovulated pseudopregnant recipient mice. Fetal weight was significantly lower after peri-implantation exposure to superovulation, regardless of preimplantation exposure (p = 0.006). Placentas derived from blastocysts exposed to superovulation pre- and peri-implantation were larger than placentas derived from natural blastocysts that are transferred into a natural or superovulated environment (p < 0.05). Fetal-to-placental weight ratio decreased following superovulation during the pre- or peri-implantation period (p = 0.05, 0.01, respectively) and these effects were additive. Peg3 DNA methylation levels were decreased in placentas derived from exposure to superovulation both pre- and peri-implantation compared with unexposed embryos and exposure of the preimplantation embryo only. Through RNA sequencing on placental tissue, changes were identified in genes involved in immune system regulation, specifically interferon signaling, which has been previously implicated in implantation and maintenance of early pregnancy in mice. Overall, we found that the timing of exposure to gonadotropin stimulation can have differential effects on fetal and placental growth. These findings could impact clinical practice and underscores the importance of dissecting the role of procedures utilized during IVF on pregnancy complications.Summary SentencePreimplantation embryo exposure to superovulation affects placental growth, whereas peri-implantation exposure affects fetal growth. Placental effects occur via changes in immune-related gene expression and epigenetic changes in growth-related genes. |
| Databáze: | OpenAIRE |
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