In-vitro activity of polyhexanide alone and in combination with antibiotics against Staphylococcus aureus
| Autor: | W. Fabry, H.-J. Kock |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus Imipenem Genotype medicine.drug_class Antibiotics Biguanides Polyhexanide Microbial Sensitivity Tests Microbiology Minimum inhibitory concentration chemistry.chemical_compound polycyclic compounds medicine Humans Minimum bactericidal concentration business.industry Broth microdilution Drug Synergism General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Molecular Typing Penicillin Infectious Diseases chemistry Linezolid Anti-Infective Agents Local business medicine.drug |
| Zdroj: | Journal of Hospital Infection. 86:68-72 |
| ISSN: | 0195-6701 |
| DOI: | 10.1016/j.jhin.2013.10.002 |
| Popis: | Summary Background The resistance of Staphylococcus aureus is increasing, not only to antibiotics but also to antiseptics. Aim To investigate the activity of the antiseptic polyhexanide and several antibiotics against clinical isolates of meticillin-susceptible and meticillin-resistant Staphylococcus aureus (MSSA and MRSA, respectively). Polyhexanide was tested alone and in combination with oxacillin, penicillin G, ampicillin, cefazolin, cefuroxime, imipenem, gentamicin, erythromycin, doxycycline, levoflocaxin, linezolid and vancomycin. Methods Fifty MSSA and 50 MRSA strains, including one vancomycin-intermediate (VISA) strain, were tested. All strains were typed by pulsed-field gel electrophoresis (PFGE) to exclude testing of clonal isolates. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined using the serial broth microdilution technique according to DIN 58940. Combinations of polyhexanide and different antibiotics were investigated using the checkerboard technique. Findings Polyhexanide MICs and MBCs in the range of 0.5–2 mg/L were found for both MSSA and MRSA, and the VISA strain had MIC and MBC values of 2 mg/L. All isolates were regarded as susceptible to polyhexanide, and no antagonism was observed between polyhexanide and the tested antibiotics. Synergism between polyhexanide and some bacteriostatic antibiotics (erythromycin, doxycycline and linezolid) was found for some strains. Conclusions Polyhexanide appears to be suitable for the topical treatment of S. aureus alone and in combination with antibiotics. |
| Databáze: | OpenAIRE |
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