G2/M checkpoint gene expression in developing germ cells
| Autor: | Suzanne Hasthorpe, Kellie M. Tainton, John M. Hutson, Kelly N. Roeszler, Martin J. Tymms, Patricia E. Lusby, Katrina M. Bell, Melissa J. Peart |
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| Rok vydání: | 2007 |
| Předmět: |
G2 Phase
Male Antimetabolites Mitosis Apoptosis Biology Mice Sex Factors Pregnancy Gene expression CDC2 Protein Kinase Testis Genetics medicine Animals Gene Adaptor Proteins Signal Transducing Gene Library Cyclin-dependent kinase 1 cDNA library Days post coitum Cell Differentiation Cell Biology Molecular biology Cell biology Up-Regulation medicine.anatomical_structure Germ Cells Animals Newborn embryonic structures Checkpoint Kinase 1 Female Fluorouracil biological phenomena cell phenomena and immunity Stem cell Signal transduction Protein Kinases Germ cell Developmental Biology |
| Zdroj: | Molecular reproduction and development. 74(5) |
| ISSN: | 1040-452X |
| Popis: | Cell cycle progression is prevented by signal transduction pathways known as checkpoints which are activated in response to replication interference and DNA damage. We cloned a G2/M cell cycle phase-related checkpoint gene from a neonatal mouse testis cDNA library which was identified as mouse claspin, a proposed adaptor protein for Chk1. As part of a study on germ cell differentiation we examined the expression of the checkpoint gene, Chk1, and claspin at 12.5 and 14.5 days post coitum (dpc) and in the post-natal phase. Chk1 mRNA expression increased from 12.5 to 14.5 dpc in female gonads and was strong in males at both time points. Claspin however, was not detected until 14.5 dpc. This suggests there may be some dissociation of claspin expression from Chk1 in fetal germ cell development. Chk1 and claspin expression was also studied in testis over the first 3 days following birth, when apoptosis regulates germ stem cell number. We modulated checkpoint-related gene expression in testis using the anti-metabolite, 5-fluorouracil, resulting in increased apoptosis and upregulation of Chk1 (P |
| Databáze: | OpenAIRE |
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