Peroxisome Proliferator-Activated Receptor γ Controls Ingestive Behavior, Agouti-Related Protein, and Neuropeptide Y mRNA in the Arcuate Hypothalamus
| Autor: | Brett J. W. Teubner, Vitaly Ryu, John T. Garretson, Timothy J. Bartness, Kevin L. Grove, Almira Vazdarjanova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Phodopus Peroxisome proliferator-activated receptor Hamster Biology Mice Internal medicine Cricetinae medicine Animals Agouti-Related Protein Neuropeptide Y RNA Messenger Receptor chemistry.chemical_classification General Neuroscience digestive oral and skin physiology Arcuate Nucleus of Hypothalamus Articles Feeding Behavior Neuropeptide Y receptor medicine.disease biology.organism_classification Mice Inbred C57BL PPAR gamma Endocrinology chemistry Hypothalamus Ghrelin Ingestive behaviors hormones hormone substitutes and hormone antagonists |
| Popis: | Peroxisome proliferator-activated receptor γ (PPARγ) is clinically targeted for type II diabetes treatment; however, rosiglitazone (ROSI), a PPARγ agonist, increases food intake and body/fat mass as side-effects. Mechanisms for these effects and the role of PPARγ in feeding are not understood. Therefore, we tested this role in Siberian hamsters, a model of human energy balance, and C57BL/6 mice. We tested the following: (1) how ROSI and/or GW9662 (2-chloro-5-nitro-N-phenylbenzamide; PPARγ antagonist) injected intraperitoneally or into the third ventricle (3V) affected Siberian hamster feeding behaviors; (2) whether food deprivation (FD) co-increases agouti-related protein (AgRP) andPPARγ mRNA expression in Siberian hamsters and mice; (3) whether intraperitoneally administered ROSI increasesAgRPandNPYinad libitum-fed animals; (4) whether intraperitoneally administered PPARγ antagonism blocks FD-induced increases inAgRPandNPY; and finally, (5) whether intraperitoneally administered PPARγ modulation affects plasma ghrelin. Third ventricular and intraperitoneally administered ROSI increased food hoarding and intake for 7 d, an effect attenuated by 3V GW9662, and also prevented (intraperitoneal) FD-induced feeding. FD hamsters and mice increasedAgRPwithin the arcuate hypothalamic nucleus with concomitant increases inPPARγ exclusively within AgRP/NPY neurons. ROSI increasedAgRPandNPYsimilarly to FD, and GW9662 prevented FD-induced increases inAgRPandNPYin both species. Neither ROSI nor GW9662 affected plasma ghrelin. Thus, we demonstrated that PPARγ activation is sufficient to trigger food hoarding/intake, increaseAgRP/NPY, and possibly is necessary for FD-induced increases in feeding andAgRP/NPY. These findings provide initial evidence that FD-induced increases inAgRP/NPYmay be a direct PPARγ-dependent process that controls ingestive behaviors. |
| Databáze: | OpenAIRE |
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