Dietary addition of rutin impairs inflammatory response and protects muscle of silver catfish (Rhamdia quelen) from apoptosis and oxidative stress in Aeromonas hydrophila-induced infection

Autor: Vanessa Medeiros da Rosa, Silvio Teixeira da Costa, Elisia Gomes da Silva, Magale Dallaporta, Karine Ariotti, Isabela Finamor, Guerino Bandeira Junior, Bernardo Baldisserotto, Agueda Castagna de Vargas, Caroline A. Bressan, Maria A. Pavanato
Rok vydání: 2019
Předmět:
0301 basic medicine
Physiology
Health
Toxicology and Mutagenesis
Rutin
chemical and pharmacologic phenomena
Apoptosis
Pharmacology
Toxicology
medicine.disease_cause
Protective Agents
Biochemistry
Antioxidants
Nitric oxide
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Fish Diseases
medicine
Animals
Catfishes
biology
Chemistry
Superoxide
Muscles
04 agricultural and veterinary sciences
Cell Biology
General Medicine
Glutathione
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Ascorbic acid
biology.organism_classification
Animal Feed
Aeromonas hydrophila
Oxidative Stress
030104 developmental biology
Dietary Supplements
040102 fisheries
biology.protein
bacteria
0401 agriculture
forestry
and fisheries
lipids (amino acids
peptides
and proteins)
Gram-Negative Bacterial Infections
Oxidative stress
Zdroj: Comparative biochemistry and physiology. Toxicologypharmacology : CBP. 226
ISSN: 1532-0456
Popis: This research aimed to assess the influence of dietary addition of rutin on inflammation, apoptosis and antioxidative responses in muscle of silver catfish (Rhamdia quelen) challenged with Aeromonas hydrophila (A. hydrophila). Fish were split into four groups as follows: control, 0.15% rutin, A. hydrophila, 0.15% rutin + A. hydrophila. After 2 weeks of feeding with standard or rutin diets, fish were challenged or not with A. hydrophila for 1 week. Rutin-added diet abrogates A. hydrophila induced-hemorrhage and inflammatory infiltration. It decreases A. hydrophila induced-apoptosis through decreasing the ratio of Bax to Bcl-2 and increasing phospho-Akt to Akt ratio. It diminishes the A. hydrophila induced-rise in nitric oxide and superoxide anion levels and reestablishes superoxide dismutase activity as well. Although such diet is unable to recover the levels of reduced glutathione (GSH), cysteine and glutamate cysteine ligase, which are depleted as a result of A. hydrophila infection, it diminishes the oxidized glutathione (GSSG) content, thus decreasing GSSG to GSH ratio. It increases the levels of cysteine residues of proteins and diminishes those of thiol-protein mixed disulfides, which were changed after A. hydrophila challenge. Finally, it reduces A. hydrophila induced-lipid peroxidation, markedly elevates ascorbic acid and thus reestablishes total antioxidant capacity, whose levels were decreased after A. hydrophila challenge. In conclusion, the dietary addition of rutin at 0.15% impairs A. hydrophila-induced inflammatory response, inhibits A. hydrophila-induced apoptosis and promotes cell survival. It also reduces the A. hydrophila-induced oxidative stress and stimulates the antioxidative responses in muscle of A. hydrophila-infected silver catfish.
Databáze: OpenAIRE
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