Efficient repopulation of genetically derived rho zero cells with exogenous mitochondria
| Autor: | Gaetano Villani, Mario Krehan, Sandra Heller, Dominga Latorre, Susanna Schubert, Peter Seibel, Martina Seibel, Ingo Schäfer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Mitochondrial DNA
lcsh:Medicine Oxidative phosphorylation Biology Mitochondrion Real-Time Polymerase Chain Reaction Genome DNA Mitochondrial Oxidative Phosphorylation Cell Fusion Electron Transport chemistry.chemical_compound Adenosine Triphosphate Cell Line Tumor Humans lcsh:Science DNA Primers Multidisciplinary Cell fusion Microscopy Confocal Base Sequence lcsh:R Wild type Transfection Molecular biology Cell biology Mitochondria chemistry lcsh:Q Adenosine triphosphate Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 9, p e73207 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Mitochondria are involved in a variety of cellular biochemical pathways among which the ATP production by oxidative phosphorylation (OXPHOS) represents the most important function of the organelle. Since mitochondria contain their own genome encoding subunits of the OXPHOS apparatus, mtDNA mutations can cause different mitochondrial diseases. The impact of these mutations can be characterized by the trans-mitochondrial cybrid technique based on mtDNA-depleted cells (ρ(0)) as acceptors of exogenous mitochondria. The aim of the present work was to compare ρ(0) cells obtained by long term ethidium bromide treatment and by a mitochondrial targeted restriction endonuclease, respectively, as mitochondrial acceptors for trans-mitochondrial cybrid generation. Fusion cells have mitochondrial respiratory functions comparable to their parental wild type cells, regardless the strategy utilized to obtain the ρ(0) acceptor cells. Therefore, the newly developed enzymatic strategy for mtDNA depletion is a more convenient and suitable tool for a broader range of applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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