Classification of type 2 diabetes rats based on urine amino acids metabolic profiling by liquid chromatography coupled with tandem mass spectrometry
| Autor: | Hongbin Zhu, Fengrui Song, Chunyan Wang, Zifeng Pi, Shuying Liu, Zhiqiang Liu |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Clinical Biochemistry Urine Type 2 diabetes Tandem mass spectrometry Biochemistry Diabetes Mellitus Experimental Analytical Chemistry Tandem Mass Spectrometry Partial least squares regression medicine Animals Cluster Analysis Amino Acids Least-Squares Analysis Rats Wistar Acarbose chemistry.chemical_classification Urine amino acids Chromatography Ms analysis Reproducibility of Results Cell Biology General Medicine medicine.disease Rats Amino acid Diabetes Mellitus Type 2 chemistry Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B. 935:26-31 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2013.07.016 |
| Popis: | An analytical method for quantifying underivatized amino acids (AAs) in urine samples of rats was developed by using liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). Classification of type 2 diabetes rats was based on urine amino acids metabolic profiling. LC–MS/MS analysis was applied through chromatographic separation and multiple reactions monitoring (MRM) transitions of MS/MS. Multivariate profile-wide predictive models were constructed using partial least squares discriminant analysis (PLS-DA) by SIMAC-P 11.5 version software package and hierarchical cluster analysis (HCA) by SPSS 18.0 version software. Some amino acids in urine of rats have significant change. The results of the present study prove that this method could perform the quantification of free AAs in urine of rats by using LC–MS/MS. In summary, the PLS-DA and HCA statistical analysis in our research were preferable to differentiate healthy rats and type 2 diabetes rats by the quantification of AAs in their urine samples. In addition, comparing with health group the seven increased amino acids in urine of type 2 rats were returned to normal under the treatment of acarbose. |
| Databáze: | OpenAIRE |
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