Effects of serum from rats with combined radiation-burn injury on the growth of hematopoietic progenitor cells
| Autor: | Xinze Ran, Huai-En Zheng, Chao-Hua Guo, Xiao-Hong Chen, Tianmin Cheng, Zhao-Wen Zong, Guoping Ai, Yongping Su |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Pathology medicine.medical_specialty Burn injury Necrosis Critical Care and Intensive Care Medicine Andrology Mice Blood serum medicine Animals Progenitor cell Rats Wistar Radiation Injuries Cells Cultured Cell Proliferation Mice Inbred BALB C business.industry Interleukin-6 Multiple Trauma Tumor Necrosis Factor-alpha Interleukin Radiation burn Radioimmunoassay medicine.disease Hematopoietic Stem Cells Rats Surgery medicine.symptom Stem cell business Burns |
| Zdroj: | The Journal of trauma. 62(1) |
| ISSN: | 0022-5282 |
| Popis: | BACKGROUND: This study aims to observe the effects of blood serum from rats with radiation injury, burn injury, and combined radiation-burn injury on the growth of hematopoietic progenitor cells and to explore the possible mechanisms. METHODS: Serum from rats with radiation injury, burn injury, and combined radiation-burn injury were collected at 3 hours, 12 hours, 24 hours, 48 hours, 72 hours, and 96 hours after injury and then was added to the culture medium to see its effect on the growth of hematopoietic progenitor cells (HPCs) at a final protein concentration of 10 microg/mL. Radioimmunoassay and enzyme-linked immunosorbent assay were employed to measure the level of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 in each group, and the effect of TNF-alpha and IL-6 on the growth of HPC was also observed. RESULTS: The number of HPCs colonies formed after addition of the serum from rats with burn or combined radiation-burn injuries was significantly higher than that from normal rats at 3 hours, 12 hours, 24 hours, 48 hours, 72 hours, and 96 hours after injury and reached its peak value at 24 hours after injury. However, fewer HPCs colonies were found after the addition of the serum from irradiated rats. At the same time, the levels of TNF-alpha and IL-6 in the serum of burn group and combined radiation-burn injury group were significantly higher than that of normal group, and much higher than that of the irradiation injury group (p < 0.01). Also, TNF-alpha and IL-6 demonstrated promoting effect on the growth of HPC. CONCLUSION: Serum from rats with burn injury and combined radiation-burn injury stimulates the growth of HPCs, while serum from irradiated rats shows inhibitory effects on the growth of HPCs. These effects may lie in the different level of TNF-alpha and IL-6 in the serum of each group. |
| Databáze: | OpenAIRE |
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