In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue

Autor: Riva de Paula Oliveira, Jean Antônio Aderaldo da Silva Filho, Raquel Brandt Giordani, Cesar Orlando Muñoz Cadavid, Samara Vitória Ferreira de Araújo, Stella Maria Andrade Gomes Barreto, Márcio Ferrari, Rafael Amir de Oliveira Moura, Hugo Alexandre Oliveira Rocha, Giovanna Melo Martins Silva
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Crops
Agricultural
Antioxidant
antioxidant
DPPH
medicine.medical_treatment
lcsh:QR1-502
Industrial Waste
02 engineering and technology
In Vitro Techniques
medicine.disease_cause
01 natural sciences
Biochemistry
Article
Antioxidants
lcsh:Microbiology
Animals
Genetically Modified
chemistry.chemical_compound
Agave
In vivo
residue
medicine
agro-industrial
Animals
Food science
Biomass
Agave sisalana
Caenorhabditis elegans
Molecular Biology
Chelating Agents
chemistry.chemical_classification
Reactive oxygen species
biology
010405 organic chemistry
Plant Extracts
Free Radical Scavengers
021001 nanoscience & nanotechnology
biology.organism_classification
In vitro
0104 chemical sciences
Plant Leaves
Oxidative Stress
chemistry
Hydroxyl radical
0210 nano-technology
Reactive Oxygen Species
Oxidative stress
Zdroj: Biomolecules, Vol 10, Iss 1435, p 1435 (2020)
Biomolecules
Volume 10
Issue 10
Popis: Agave sisalana agro-industrial residue has considerable potential against damage associated with oxidative stress and skin aging. This study aims to demonstrate, in vitro and in vivo, the potential of Agave sisalana agro-industrial residue as a safe and effective alternative for the prevention of damage caused by oxidative stress and aging. The antioxidant activity was evaluated in vitro (total antioxidant capacity, reducing power, DPPH radical scavenging, metal chelating (Fe2+ and Cu2+), and hydroxyl radical scavenging) and in vivo using the Caenorhabditis elegans organism model. The extract showed in vitro antioxidant activity in all tests performed. Tests with C. elegans showed that the extract was able to reduce the intracellular levels of reactive oxygen species (ROS) and increase the survival rate of worms. A downregulation of gst-4::GFP expression suggests a direct action against free radicals. Agave sisalana agro-industrial residue extract (AsRE) can therefore be considered as a source of antioxidant biomolecules, and the use of this agro-industrial residue in a new production process can lead to sustainability and socioeconomic development.
Databáze: OpenAIRE
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