The soluble pool of HLA-G produced by human trophoblasts does not include detectable levels of the intron 4-containing HLA-G5 and HLA-G6 isoforms
| Autor: | Gernot Desoye, Gottfried Dohr, Herbert Juch, Astrid Blaschitz, A. Volz, Heinz Hutter, C. Daxboeck |
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| Rok vydání: | 2005 |
| Předmět: |
Gene isoform
Embryology Placenta Syncytiotrophoblasts Biology Cell Line Western blot HLA Antigens Pregnancy Fetal membrane HLA-G Genetics medicine Humans Protein Isoforms Molecular Biology reproductive and urinary physiology HLA-G Antigens Labor Obstetric medicine.diagnostic_test Histocompatibility Antigens Class I Antibodies Monoclonal Obstetrics and Gynecology Cell Biology Molecular biology Trophoblasts Blot Alternative Splicing Pregnancy Trimester First medicine.anatomical_structure Reproductive Medicine embryonic structures Female Cytotrophoblasts Protein Processing Post-Translational Biomarkers Developmental Biology |
| Zdroj: | Molecular Human Reproduction. 11:699-710 |
| ISSN: | 1460-2407 1360-9947 |
| DOI: | 10.1093/molehr/gah185 |
| Popis: | In the context of implantation and pregnancy, several immunomodulating functions have been attributed to the different HLA-G isoforms. Increasing attention is now being addressed to the actively secreted soluble forms, because they might have a systemic function or could be useful as diagnostic tools. However, the cellular source of secretion, even during pregnancy, where HLA-G expression level is known to be highest, is still under debate. To elucidate the conflicting results, we investigated the isoform distribution in human first trimester and term placentas in situ and in vitro. Results obtained by applying immunohistochemistry, western blot, enzyme-linked immunosorbent assay (ELISA) and RT-PCR show that (1) all of the alpha1 domain-containing HLA-G isoforms are restrictedly expressed in the extravillous cytotrophoblasts (EVCTs) and very few first-trimester syncytiotrophoblasts, which directly cover cell columns, whereas mesenchymal cells of the villous chorion do not express HLA-G; (2) as demonstrated in western blots, trophoblasts express only the HLA-G1 isoform; (3) HLA-G5 and -G6 transcripts could be detected in human term placenta and isolated first-trimester trophoblasts but levels are extremely low; and (4) conditioned media of primary first-trimester trophoblasts, and the chorion laeve-derived trophoblastic cell line AC1-M59 do contain HLA-G1 fragments shed from the cell surface. Our data provide substantial evidence that none of the intron 4-containing isoforms, the so-called actively secreted, soluble HLA-G5 or -G6, are produced by human trophoblasts in situ or in vitro. |
| Databáze: | OpenAIRE |
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