Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals
| Autor: | Kiichi Watanabe, Shigenobu Yonemura, Ayaka Nishiyama, Mami Matsuo-Takasaki, Takafumi Wataya, Mototsugu Eiraku, Masako Kawada, Michiru Matsumura, Keiko Muguruma, Yoshiki Sasai |
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| Rok vydání: | 2008 |
| Předmět: |
Fibroblast Growth Factor 8
Cell Bone Morphogenetic Protein 4 Biology Fibroblast growth factor Tissue Culture Techniques Wnt3 Protein Mice Genetics medicine Animals Homeostasis Humans Embryonic Stem Cells Body Patterning Cerebral Cortex Neurons Cell Cycle Wnt signaling pathway Gene Expression Regulation Developmental Cell Differentiation Cell Biology Anatomy Antigens Differentiation Immunohistochemistry STEMCELL Olfactory bulb Cell biology Wnt Proteins Corticogenesis medicine.anatomical_structure Active manipulation Molecular Medicine Choroid plexus Signal Transduction Cerebral organoid |
| Zdroj: | Cell Stem Cell. 3:519-532 |
| ISSN: | 1934-5909 |
| DOI: | 10.1016/j.stem.2008.09.002 |
| Popis: | Summary Here, we demonstrate self-organized formation of apico-basally polarized cortical tissues from ESCs using an efficient three-dimensional aggregation culture (SFEBq culture). The generated cortical neurons are functional, transplantable, and capable of forming proper long-range connections in vivo and in vitro. The regional identity of the generated pallial tissues can be selectively controlled (into olfactory bulb, rostral and caudal cortices, hem, and choroid plexus) by secreted patterning factors such as Fgf, Wnt, and BMP. In addition, the in vivo-mimicking birth order of distinct cortical neurons permits the selective generation of particular layer-specific neurons by timed induction of cell-cycle exit. Importantly, cortical tissues generated from mouse and human ESCs form a self-organized structure that includes four distinct zones (ventricular, early and late cortical-plate, and Cajal-Retzius cell zones) along the apico-basal direction. Thus, spatial and temporal aspects of early corticogenesis are recapitulated and can be manipulated in this ESC culture. |
| Databáze: | OpenAIRE |
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