3-Phosphoinositide-dependent protein kinase-1/Akt signalling and inhibition in a canine prostate carcinoma cell line

Autor:	Thomas J. Rosol, William C. Kisseberth, Ching-Shih Chen, Samuel K. Kulp, Francisco J. Alvarez, C. G. Couto, Sridhar Murahari
Rok vydání:	2009
Předmět:	medicine.medical_specialty General Veterinary Chemistry Cancer OSU-03012 medicine.disease Prostate cancer chemistry.chemical_compound Canine Prostate Carcinoma Endocrinology Internal medicine medicine Cancer research Phosphorylation Protein kinase A Protein kinase B PI3K/AKT/mTOR pathway
Zdroj:	Veterinary and comparative oncology. 5(1)
ISSN:	1476-5829
Popis:	Deregulation of the 3-phosphoinositide-dependent protein kinase-1 (PDK-1)/Akt signalling pathway is associated with prostate cancer development and progression. Inhibition of PDK-1/Akt signalling can be achieved using structurally optimized celecoxib derivatives such as OSU-03012. In this study, we treated the novel canine prostate cancer cell line, Ace-1, with OSU-03012 or dimethyl sulphoxide in vitro . We found that Akt was constitutively phosphorylated in the canine prostate cancer cell line Ace-1 and that there was a dose-dependent decrease in cell viability, and Akt and glycogen synthase kinase-3 phosphorylation, in response to OSU-03012 treatment. This was accompanied by a dose-dependent increase in apoptosis. These data suggest that Akt signalling pathway inhibition is a potential strategy for the treatment of dogs with prostate cancer and that canine prostate cancer is a relevant large animal model for evaluating Akt pathway inhibitors such as OSU-03012 for use in people.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43e3ce33864b55b0f28ee1b2e75c1915 https://pubmed.ncbi.nlm.nih.gov/19754801 Zobrazit plný text záznamu Plný text