Cytokine expression by neutrophils and macrophages in vivo: endotoxin induces tumor necrosis factor-alpha, macrophage inflammatory protein-2, interleukin-1 beta, and interleukin-6 but not RANTES or transforming growth factor-beta 1 mRNA expression in acute lung inflammation
| Autor: | Haresh Kirpalani, Zhou Xing, Manel Jordana, T. J. Schall, Jack Gauldie, K. E. Driscoll |
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| Rok vydání: | 1994 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lipopolysaccharides Neutrophils medicine.medical_treatment Clinical Biochemistry Chemokine CXCL2 Inflammation Biology Rats Sprague-Dawley Transforming Growth Factor beta medicine Macrophage Animals Northern blot RNA Messenger Interleukin 6 Molecular Biology medicine.diagnostic_test Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Monokines Interleukin Cell Biology Pneumonia Blotting Northern Molecular biology Rats Disease Models Animal Kinetics Bronchoalveolar lavage Cytokine Immunology Acute Disease biology.protein Cytokines Tumor necrosis factor alpha medicine.symptom Bronchoalveolar Lavage Fluid Interleukin-1 |
| Zdroj: | American journal of respiratory cell and molecular biology. 10(2) |
| ISSN: | 1044-1549 |
| Popis: | Using a rat model of acute lung inflammation induced by intratracheal instillation of lipopolysaccharide (LPS), we investigated the kinetics of mRNA expression and the potential cellular sources of tumor necrosis factor-alpha (TNF-alpha), macrophage inflammatory protein-2 (MIP-2), interleukin (IL)-1 beta, IL-6, RANTES, and transforming growth factor-beta 1 (TGF-beta 1). By Northern blot analysis, TNF-alpha and MIP-2 mRNAs in total lung tissue increased markedly by 30 min and peaked by 1 h after LPS exposure, whereas expression of IL-1 beta and IL-6 was not detected until 1 h and peaked within 6 h. In contrast, neither RANTES nor TGF-beta 1 mRNA was induced by LPS throughout 72 h, although a basal expression was detected in both saline- and LPS-treated lung tissues. At 1 h after LPS, the bronchoalveolar lavage (BAL) fluid contained about 98% alveolar macrophages (AM), whereas by 6 or 12 h, 88% of BAL cells were polymorphonuclear neutrophils (PMN). Upon extraction of total RNA after separation of AM from PMN in BAL, Northern analysis showed that at 1 h, AM expressed pronounced signals for TNF-alpha, MIP-2, IL-1 beta, and IL-6. At 6 and 12 h, however, while cytokine transcripts decreased in AM, PMN exhibited strong signals for these cytokines. A low basal noninducible signal for TGF-beta 1 but not RANTES was detected in both AM and PMN. Finally, by in situ hybridization techniques, PMN in the lung tissue, particularly those located in the vicinity of the bronchiole and vasculature, were demonstrated to localize MIP-2 mRNA.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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