Identification of two nogo/rtn4 genes and analysis of Nogo-A expression in Xenopus laevis

Autor: Martin E. Schwab, Thomas Oertle, Cordula Hirsch, Claudia A. O. Stuermer, Heike Diekmann, Michael Klinger, Dietmar Heinz, Barbara Petrausch, Sylvia Hannbeck von Hanwehr
Rok vydání: 2004
Předmět:
Central Nervous System
Nervous system
Gene isoform
DNA
Complementary
Nogo Proteins
Molecular Sequence Data
Central nervous system
Xenopus
Hindbrain
Nerve Fibers
Myelinated
Xenopus laevis
03 medical and health sciences
Cellular and Molecular Neuroscience
Myelin
0302 clinical medicine
Sequence Homology
Nucleic Acid
ddc:570
Neural Pathways
mental disorders
medicine
Animals
Amino Acid Sequence
Axon
Molecular Biology
Spinal Cord Regeneration
030304 developmental biology
0303 health sciences
Base Sequence
Sequence Homology
Amino Acid
biology
Gene Expression Regulation
Developmental
Membrane Proteins
Cell Differentiation
Cell Biology
biology.organism_classification
Cell biology
medicine.anatomical_structure
Rabbits
Neuroscience
Myelin Proteins
psychological phenomena and processes
030217 neurology & neurosurgery
Zdroj: Molecular and Cellular Neuroscience. 25:205-216
ISSN: 1044-7431
DOI: 10.1016/j.mcn.2003.09.021
Popis: Myelin-associated axon growth inhibitors such as Nogo-A/RTN4-A impair axon regeneration in the adult mammalian central nervous system (CNS). Here, we describe the cloning and expression of two independent Xenopus laevis rtn4 orthologs. As in mammals, alternative transcripts are generated both through differential splicing and promoter usage, giving rise to Xenopus nogo-A, -B, -C and to a new isoform, nogo-N/rtn4-N. Xenopus is therefore the ‘lowest’ vertebrate where Nogo-A was identified. Xenopus Nogo-A/RTN4-A is predominantly expressed in the nervous system, whereas the other isoforms mainly occur in nonneuronal tissues. Nogo-A/RTN4-A specific antisera detect the protein in myelinated fiber tracts of the spinal cord, hindbrain, optic nerve, tectum opticum and in isolated oligodendrocytes. In addition, subpopulations of CNS neurons are Nogo-A/RTN4-A positive. This expression pattern is consistent with that observed for rat Nogo-A and suggests similar functions. Nogo-A in Xenopus myelin might therefore contribute to the failure of spinal cord regeneration in frogs—a feature that may have evolved during the transition from fish to land vertebrates.
Databáze: OpenAIRE
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