Comprehensive Analysis of the Alternaria Mycobolome Using Mass Spectrometry Based Metabolomics
| Autor: | Richard Hammerl, Stefan Asam, Basem Kanawati, Philippe Schmitt-Kopplin, Thomas Hofmann, Oliver Frank, Michael Rychlik, Marina Gotthardt, Frank R. Schmidt |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Alternaria Alternata Alterperylenepoxide A-9-mercaptolactate Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Non-targeted Metabolomics Ultra-high Resolution Secondary Metabolism Biology Secondary metabolite Alternaria alternata Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Metabolomics Tandem Mass Spectrometry medicine Tenuazonic acid Metabolome ddc:630 Food science Mycotoxin 030109 nutrition & dietetics food and beverages Alternaria Mycotoxins biology.organism_classification ddc 030104 developmental biology chemistry Tentoxin Food Science Biotechnology medicine.drug Chromatography Liquid |
| Zdroj: | Mol. Nutr. Food Res. 64:1900558 (2020) |
| ISSN: | 1613-4133 |
| Popis: | Scope Alternaria fungi are widely distributed plant pathogens infecting grains and vegetables and causing major harvest losses in the field and during postharvest storage. Besides, consumers are endangered by the formation of toxic secondary metabolites. Some of these secondary metabolites are chemically characterized as mycotoxins, but the majority of the Alternaria mycobolome still remains unknown. Methods and results Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) and LC-MS/MS are combined for the non-targeted and targeted analysis of the metabolome of three A. alternata isolates and one A. solani isolate. Due to the ultra-high resolution of FTICR-MS, unique molecular formulae are assigned to measured m/z signals. The molecular formulae are matched to entries of the databases Antibase and Kyoto Encyclopedia of Genes and Genomes. The non-targeted analysis of the fungal extracts reveals variations in the secondary metabolite profile of A. alternata and A. solani. Differences in the biosynthesis of dibenzo-α-pyrones, perylene quinones, tentoxin, and tenuazonic acid of the A. alternata and A. solani isolates are determined applying targeted LC-MS/MS. Conclusion FTICR-MS analyses reveal clear differences in the metabolic profile of the A. solani and the A. alternata isolates. |
| Databáze: | OpenAIRE |
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