The Impaired Viability of Prostate Cancer Cell Lines by the Recombinant Plant Kallikrein Inhibitor*
| Autor: | Edgar J. Paredes-Gamero, Joana Gasperazzo Ferreira, Erika Suzuki, Paula Malloy Motta Diniz, Amanda Nogueira-Pedro, Thaysa Paschoalin, Maria Luiza Vilela Oliva, Yara Aparecida Lobo, Claudia Alessandra Andrade de Paula, Paloma Korehisa Maza, Marcos S. Toledo |
|---|---|
| Přispěvatelé: | Universidade Federal de São Paulo (UNIFESP) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
Male Angiogenesis Cell Survival Angiogenesis Inhibitors Apoptosis Biology urologic and male genital diseases Biochemistry Prostate cancer DU145 immune system diseases Cell Movement Cell Line Tumor medicine Cell Adhesion Human Umbilical Vein Endothelial Cells Humans Viability assay cardiovascular diseases Calcium Signaling neoplasms Molecular Biology Plant Proteins urogenital system Caspase 3 Cancer Cytochromes c Prostatic Neoplasms Cell Biology Cell Cycle Checkpoints Cell cycle Fibroblasts medicine.disease Antineoplastic Agents Phytogenic biological factors Caspase 9 Recombinant Proteins Cell biology Mitochondria Endothelial stem cell Kallikreins Trypsin Inhibitor Kunitz Soybean Hydrophobic and Hydrophilic Interactions circulatory and respiratory physiology |
| Zdroj: | Repositório Institucional da UNIFESP Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| Popis: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Prostate cancer is the most common type of cancer, and kallikreins play an important role in the establishment of this disease. rBbKIm is the recombinant Bauhinia bauhinioides kallikreins inhibitor that was modified to include the RGD/RGE motifs of the inhibitor BrTI from Bauhinia rufa. This work reports the effects of rBbKIm on DU145 and PC3 prostate cancer cell lines. rBbKIm inhibited the cell viability of DU145 and PC3 cells but did not affect the viability of fibroblasts. rBbKIm caused an arrest of the PC3 cell cycle at the G0/G1 and G2/M phases but did not affect the DU145 cell cycle, although rBbKIm triggers apoptosis and cytochrome c release into the cytosol of both cell types. the differences in caspase activation were observed because rBbKIm treatment promoted activation of caspase-3 in DU145 cells, whereas caspase-9 but not caspase-3 was activated in PC3 cells. Because angiogenesis is important to the development of a tumor, the effect of rBbKIm in this process was also analyzed, and an inhibition of 49% was observed in in vitro endothelial cell capillary-like tube network formation. in summary, we demonstrated that different properties of the protease inhibitor rBbKIm may be explored for investigating the androgen-independent prostate cancer cell lines PC3 and DU145. Universidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04044020 São Paulo, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044020 São Paulo, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04044020 São Paulo, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044020 São Paulo, Brazil FAPESP: 2009/53766-5 Web of Science |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|