Comparative plastome analysis of Blumea , with implications for genome evolution and phylogeny of Asteroideae
| Autor: | Parviz Heidari, Abdullah, Ibrar Ahmed, Péter Poczai, Furrukh Mehmood, Abdur Rahim |
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| Přispěvatelé: | Botany, Embryophylo |
| Rok vydání: | 2021 |
| Předmět: |
DYNAMICS
0106 biological sciences CHLOROPLAST GENOMES ORGANIZATION Asteraceae SEQUENCE 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Gnaphalieae Anthemideae oligonucleotide repeat QH540-549.5 Ecology Evolution Behavior and Systematics 030304 developmental biology Nature and Landscape Conservation Asteroideae 0303 health sciences Ecology biology TRANSFER-RNA GENES 1184 Genetics developmental biology physiology barcode LEVEL PHYLOGENY DNA Calenduleae Senecioneae 15. Life on land 11831 Plant biology plastome biology.organism_classification ALIGNMENT Inuleae Evolutionary biology polymorphic loci Blumea 1181 Ecology evolutionary biology MATERNAL INHERITANCE Astereae |
| Zdroj: | Ecology and Evolution, Vol 11, Iss 12, Pp 7810-7826 (2021) |
| ISSN: | 2045-7758 |
| DOI: | 10.1002/ece3.7614 |
| Popis: | The genus Blumea (Asteroideae, Asteraceae) comprises about 100 species, including herbs, shrubs, and small trees. Previous studies have been unable to resolve taxonomic issues and the phylogeny of the genus Blumea due to the low polymorphism of molecular markers. Therefore, suitable polymorphic regions need to be identified. Here, we de novo assembled plastomes of the three Blumea species B. oxyodonta, B. tenella, and B. balsamifera and compared them with 26 other species of Asteroideae after correction of annotations. These species have quadripartite plastomes with similar gene content, genome organization, and inverted repeat contraction and expansion comprising 113 genes, including 80 protein‐coding, 29 transfer RNA, and 4 ribosomal RNA genes. The comparative analysis of codon usage, amino acid frequency, microsatellite repeats, oligonucleotide repeats, and transition and transversion substitutions has revealed high resemblance among the newly assembled species of Blumea. We identified 10 highly polymorphic regions with nucleotide diversity above 0.02, including rps16‐trnQ, ycf1, ndhF‐rpl32, petN‐psbM, and rpl32‐trnL, and they may be suitable for the development of robust, authentic, and cost‐effective markers for barcoding and inference of the phylogeny of the genus Blumea. Among these highly polymorphic regions, five regions also co‐occurred with oligonucleotide repeats and support use of repeats as a proxy for the identification of polymorphic loci. The phylogenetic analysis revealed a close relationship between Blumea and Pluchea within the tribe Inuleae. At tribe level, our phylogeny supports a sister relationship between Astereae and Anthemideae rooted as Gnaphalieae, Calenduleae, and Senecioneae. These results are contradictory to recent studies which reported a sister relationship between “Senecioneae and Anthemideae” and “Astereae and Gnaphalieae” or a sister relationship between Astereae and Gnaphalieae rooted as Calenduleae, Anthemideae, and then Senecioneae using nuclear genome sequences. The conflicting phylogenetic signals observed at the tribal level between plastidt and nuclear genome data require further investigation. |
| Databáze: | OpenAIRE |
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