Identification of a Glycerophosphocholine Phosphodiesterase, GDE5, in chicken
| Autor: | Hao Gao, Pingan Chang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Glycerophosphodiester phosphodiesterase chemistry.chemical_classification Messenger RNA Glycerophosphocholine phosphodiesterase Chemistry Lipid metabolism Amino acid Environmental sciences 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Glycerophosphocholine phosphodiesterase activity Biochemistry Cytoplasm 030220 oncology & carcinogenesis Complementary DNA GE1-350 |
| Zdroj: | E3S Web of Conferences, Vol 245, p 03054 (2021) |
| ISSN: | 2267-1242 |
| Popis: | Glycerophosphodiester phosphodiesterase (GDPD/GDE) catalyzes the hydrolysis of glycerophosphodiesters to glycerol 3-phosphate and alcohol. It was discovered that the glycerophosphodiesterase family plays a role in lipid metabolism and signal pathway in recent years, but little has been known about the characteristics of chicken GDEs. Here, chicken GDE5 (cGDE5) was identified and characterized for the first time. The full length coding cDNA sequence of cGDE5 was cloned, which encoded a polypeptide with 678 amino acids containing a carbohydrate-binding module 20 (CBM20) and a GDPD domain. Tissue expression profiles showed that cGDE5 mRNA was high in various tissues. such as heart, brain, skeletal muscle and testis. Moreover, cGDE5 was demonstrated to exhibit glycerophosphocholine phosphodiesterase activity. These results together suggested that cGDE5, as a unique member of GDE family, may play multiple roles as a cytoplasmic glycerophosphocholine phosphodiesterase. |
| Databáze: | OpenAIRE |
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