Is shorter transient middle cerebral artery occlusion (t-MCAO) duration better in stroke experiments on diabetic female Sprague Dawely rats?
| Autor: | Mihael Mišir, Marija Renic, Ines Drenjančević, Martina Mihalj, Sanja Novak |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Blood Glucose
Time Factors Diabetic rat Neuroscience (miscellaneous) Ischemia 030204 cardiovascular system & hematology Statistics Nonparametric Diabetes Mellitus Experimental Rats Sprague-Dawley Model cerebral ischaemia diabetes female middle cerebral artery occlusion rats stroke transient 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Ischaemic stroke Developmental and Educational Psychology medicine Laser-Doppler Flowmetry Animals cardiovascular diseases Middle cerebral artery occlusion Stroke business.industry Infarction Middle Cerebral Artery Cerebral Infarction Fasting medicine.disease Highly sensitive Rats Disease Models Animal Cerebral blood flow Anesthesia Cerebrovascular Circulation Female Neurology (clinical) business 030217 neurology & neurosurgery |
| Zdroj: | Brain injury. 30(11) |
| ISSN: | 1362-301X |
| Popis: | AIM: To determine optimal duration of transient middle cerebral artery occlusion (t-MCAO) for a stroke model in female diabetic Sprague-Dawley (SD) rats. METHODS: Streptozotocin-induced type-1 diabetic SD female rats (n = 25, 12 weeks old, five groups ; n = 5 per group) were subjected to different duration of t-MCAO (20, 30, 45, 60 and 90 minutes) followed by reperfusion. A control group of rats without diabetes (n = 5) was subjected to 30 minutes of t-MCAO followed by reperfusion. Twenty-four hours after reperfusion, infarct volumes were evaluated by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. RESULTS: Intra- ischaemic reductions of regional cerebral blood flow (rCBF) were similar in all groups (68-75% of baseline values). Reperfusion was significantly impaired in the 90-minute ischaemia group (56-62% vs 80-125% in other groups). Twenty minutes of t-MCAO induced a small infarct (3 ± 5% of ischaemic hemisphere). Thirty minutes of ischaemia produced a significantly larger infarct (46 ± 6%). In the 45 and 60 minute groups, ischaemia infarct was 52 ± 5% and 59 ± 3% of the ischaemic hemisphere, respectively. Ischaemia of 90' led to a massive stroke (89 ± 6% of ischaemic hemisphere encompassing the whole striatum (22 ± 3%) and almost the whole MCA irrigated cortex area (67 ± 6%)). Thirty minutes of t-MCAO did not produce stroke in the control group. CONCLUSION: The diabetic rat stroke model should be different from the non-diabetic, because female type-1 diabetic SD rats are highly sensitive to brain ischaemia and it is necessary to significantly shorten the duration of t-MCAO, optimally to 30 minutes. |
| Databáze: | OpenAIRE |
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