Single-Cell Sequencing of T cell Receptors: A Perspective on the Technological Development and Translational Application
| Autor: | Cuong Q. Nguyen, Shivai Gupta, Touyana Semenova, Alexandria Voigt, Richard Witas |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
biology
Effector T-Lymphocytes Cell T-cell receptor Histocompatibility Antigens Class I Receptors Antigen T-Cell Context (language use) chemical and pharmacologic phenomena Autoimmunity Computational biology medicine.disease_cause Major histocompatibility complex Article medicine.anatomical_structure Single cell sequencing Antigen medicine biology.protein Animals Humans Single-Cell Analysis Sequence Analysis |
| Zdroj: | Adv Exp Med Biol Advances in Experimental Medicine and Biology ISBN: 9789811544934 |
| ISSN: | 0065-2598 |
| Popis: | T cells recognize peptides bound to major histocompatibility complex (MHC) class I and class II molecules at the cell surface. This recognition is accomplished by the expression of T cell receptors (TCR) which are required to be diverse and adaptable in order to accommodate the various and vast number of antigens presented on the MHCs. Thus, determining TCR repertoires of effector T cells is necessary to understand the immunological process in responding to cancer progression, infection, and autoimmune development. Furthermore, understanding the TCR repertoires will provide a solid framework to predict and test the antigen which is more critical in autoimmunity. However, it has been a technical challenge to sequence the TCRs and provide a conceptual context in correlation to the vast number of TCR repertoires in the immunological system. The exploding field of single-cell sequencing has changed how the repertoires are being investigated and analyzed. In this review, we focus on the biology of TCRs, TCR signaling and its implication in autoimmunity. We discuss important methods in bulk sequencing of many cells. Lastly, we explore the most pertinent platforms in single-cell sequencing and its application in autoimmunity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|