STAT3 influences the characteristics of stem cells in cervical carcinoma
| Autor: | Ming‑Qun Li, Hong-Ying Ren, Ping Jia, Shu-Wei Li, Hua Wang, Jie Deng, Qing-Hong Zhou, Wei Zhang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Homeobox protein NANOG Cancer Research Biology medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Western blot SOX2 Cancer stem cell medicine medicine.diagnostic_test Oncogene Articles Squamous carcinoma stem cell 030104 developmental biology Oncology 030220 oncology & carcinogenesis embryonic structures signal transducer and activator of transcription 3 Cancer research cervical carcinoma biological phenomena cell phenomena and immunity Stem cell Carcinogenesis |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2017.6454 |
| Popis: | The purpose of the study was to investigate the role of the signal transducer and activator of transcription 3 (STAT3), signal transduction protein in regulating the biological characteristics of stem cells in cervical carcinoma. Overexpressed plasmid of STAT3 was constructed and used to transfect SiHa into cervical carcinoma cells. STAT3-targeted specific siRNA was designed and produced. The effects of STAT3 upregulation (or inhibition) on the expression of NANOG, OCT4 and SOX2 markers of stem cells were measured, using western blot analysis and RT-qPCR. In addition, the tumor sphere experiment was also conducted to detect the formation of tumor spheres after the intervention of expression of STAT3 and the expression of STAT3, NANOG, OCT4 and SOX2 was detected in 35 cases of cervical carcinoma tissues and 31 cases of normal cervical tissues using immunohistochemistry. We determined whether the STAT3 overexpression plasmid was successfully constructed using enzyme digestion, PCR for bacterium solution, western blot analysis and RT-qPCR and found that the plasmid met the requirements of subsequent procedures. Compared with the empty plasmid group and STAT3 low expression group, the mRNA and protein expression of markers of stem cells, OCT4, SOX2 and NANOG were significantly elevated in the STAT3 overexpression group with statistically significant differences (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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