Endotoxic activity of lipopolysaccharides isolated from emergent potential cystic fibrosis pathogens
| Autor: | Ian R. Poxton, Emma C Bonell, John R. W. Govan, Michael L. Hutchison |
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| Rok vydání: | 2000 |
| Předmět: |
Lipopolysaccharides
Microbiology (medical) Cystic Fibrosis Lipopolysaccharide medicine.drug_class Immunology Antibiotics Enzyme-Linked Immunosorbent Assay medicine.disease_cause Microbiology Cystic fibrosis Cell Line Proinflammatory cytokine chemistry.chemical_compound Gram-Negative Bacteria medicine Humans Immunology and Allergy Respiratory Tract Infections biology Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Interleukin-8 Pathogenic bacteria General Medicine Achromobacter xylosoxidans Antimicrobial biology.organism_classification medicine.disease Endotoxins Stenotrophomonas maltophilia Infectious Diseases chemistry Cytokines Gram-Negative Bacterial Infections |
| Zdroj: | FEMS Immunology & Medical Microbiology. 27:73-77 |
| ISSN: | 1574-695X 0928-8244 |
| DOI: | 10.1111/j.1574-695x.2000.tb01414.x |
| Popis: | Improved antimicrobial therapies against the classical spectrum of pathogenic bacteria which colonise the lungs of cystic fibrosis (CF) patients has resulted in improved life expectancy and quality of life. Bacterial species that are resistant to a broad range of antibiotics including Stenotrophomonas maltophilia and Alcaligenes xylosoxidans have now emerged as potential new pathogens to fill the niche. At present, it is unclear from clinical data whether these microbes are commensal or pathogenic. In this study we have quantified the inflammatory potential of lipopolysaccharide (LPS) from eight species of Gram-negative organisms which have been cultured with increasing frequency from CF patients. Inflammatory responses induced by LPS from whole human blood and a human-derived monocyte cell line (THP-1) were assessed. Enzyme-linked immunosorbent assays were used to detect interleukin-6, interleukin-8, and tumour necrosis factor alpha (TNF). A bioassay was also used to assess TNF activity. With the exception of S. maltophilia, LPS extracted from all of the bacteria tested upregulated, by varying degrees, expression of each of the proinflammatory cytokines assayed. This study represents the first comprehensive report of the endotoxic potential of a new wave of microbes which are associated with CF. |
| Databáze: | OpenAIRE |
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