MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)
| Autor: | Xiaoliu Zhou, Xuejun Zhou, Zhencai Xu, Junwei Yang, Chengliang Xing, Zhiqun Li, Jiajun Huang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Carcinogenesis
nasopharyngeal cancer Bioengineering medicine.disease_cause Applied Microbiology and Biotechnology Downregulation and upregulation Cell Line Tumor miR-150 microRNA medicine Humans RNA Neoplasm Viability assay Chemistry Cell growth Nasopharyngeal Neoplasms General Medicine PYCR1 Neoplasm Proteins body regions MicroRNAs Real-time polymerase chain reaction Hsa-miR-150-5p Apoptosis embryonic structures Cancer research Pyrroline Carboxylate Reductases TP248.13-248.65 Research Article Research Paper Biotechnology |
| Zdroj: | Bioengineered article-version (VoR) Version of Record Bioengineered, Vol 12, Iss 2, Pp 9766-9778 (2021) |
| ISSN: | 2165-5987 2165-5979 |
| DOI: | 10.1080/21655979.2021.1995102 |
| Popis: | Nasopharyngeal cancer is a rare cancer type, but with a low five-year survival rate. Dysregulation of pyrroline-5-carboxylate reductase 1 (PYCR1) and microRNA hsa-miR-150-5p is involved in the development of various cancers. However, the molecular mechanism of the hsa-miR-150-5p-PYCR1 axis in nasopharyngeal cancer remains unclear. To identify the mechanism of the hsa-miR-150-5p-PYCR1 axis, the expression of hsa-miR-150-5p and PYCR1 in nasopharyngeal cancer tissues and cells was first measured by reverse transcription quantitative polymerase chain reaction. The luciferase and RNA pull-down assays were used to confirm the interaction between hsa-miR-150-5p and PYCR1. The overexpression of hsa-miR-150-5p and PYCR1 was detected by cell viability, proliferation, western blotting, migration, and invasion in nasopharyngeal cancer cells. The expression levels of hsa-miR-150-5p was reduced in the nasopharyngeal cancer tissues and cells and were negatively correlated with the PYCR1 levels. The upregulation of hsa-miR-150-5p significantly repressed cell growth and promoted apoptosis. However, the upregulation of PYCR1 expression significantly promoted nasopharyngeal carcinogenesis, which could abolish the inhibitory effect of hsa-miR-150-5p. In conclusion, we clarified that hsa-miR-150-5p attenuated nasopharyngeal carcinogenesis by reducing the PYCR1 expression levels. This provides a new perspective of nasopharyngeal cancer involving both hsa-miR-150-5p and PYCR1 for the treatment of nasopharyngeal cancer. |
| Databáze: | OpenAIRE |
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