Genotoxicity and Fetal Abnormality in Streptozotocin-Induced Diabetic Rats Exposed to Cigarette Smoke Prior to and During Pregnancy
| Autor: | M. S. S. Souza, Isabela Lovizutto Iessi, Marilza Vieira Cunha Rudge, Gustavo Tadeu Volpato, Ana Carolina Inhasz Kiss, Mariana Takaku, Iracema de Mattos Paranhos Calderon, Yuri Karen Sinzato, P. H. O. Lima, Débora Cristina Damasceno |
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| Rok vydání: | 2011 |
| Předmět: |
Atmosphere Exposure Chambers
Offspring Endocrinology Diabetes and Metabolism Pregnancy in Diabetics Physiology Streptozocin Tobacco smoke Diabetes Mellitus Experimental Fetal Development Toxicology Random Allocation Fetus Endocrinology Pregnancy Diabetes mellitus Leukocytes Internal Medicine medicine Animals Rats Wistar Sidestream smoke business.industry Smoking Abnormalities Drug-Induced General Medicine medicine.disease Streptozotocin Rats Comet assay Maternal Exposure Hyperglycemia Female Tobacco Smoke Pollution Comet Assay business DNA Damage medicine.drug |
| Zdroj: | Experimental and Clinical Endocrinology & Diabetes. 119:549-553 |
| ISSN: | 1439-3646 0947-7349 |
| Popis: | BACKGROUND Maternal hyperglycemia during early pregnancy is associated with increased risk of abnormalities in the offspring. Malformation rates among the offspring of diabetic mothers are 2-5-fold higher than that of the normal population, and congenital malformations are the major cause of mortality and morbidity in the offspring of diabetic mothers. Metabolic changes, such as hyperglycemia and the metabolites obtained from cigarettes both increase the production of reactive oxygen species (ROS) in the embryo or fetus, causing DNA damage. OBJECTIVE To evaluate the maternal and fetal genotoxicity, and to assess the incidence of fetal anomaly in diabetic female rats exposed to cigarette smoke at different stages of pregnancy in rats. MATERIAL AND METHOD Diabetes was induced by streptozotocin administration and cigarette smoke exposure was produced by a mechanical smoking device that generated mainstream smoke that was delivered into a chamber. Female Wistar rats were randomly assigned to: non-diabetic (ND) and diabetic (D) groups exposed to filtered air; a diabetic group exposed to cigarette smoke prior to and during pregnancy (DS) and a diabetic group only exposed to cigarette smoke prior to pregnancy (DSPP). On pregnancy day 21, blood samples were obtained for DNA damage analysis and fetuses were collected for congenital anomaly assessment. Statistical significance was set at p |
| Databáze: | OpenAIRE |
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