Molecular evidence supporting the neoplastic nature of odontogenic keratocyst: a laser capture microdissection study of 15 cases
| Autor: | Don-John Summerlin, Charles E. Tomich, Henley Jd, Liang Cheng, Shaobo Zhang |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Genetic Markers
Pathology medicine.medical_specialty Histology Loss of Heterozygosity Biology Pathology and Forensic Medicine Loss of heterozygosity Odontogenic cyst Gene Frequency Chromosome regions medicine Humans Mandibular Diseases Keratocyst Microdissection Alleles Laser capture microdissection Chromosomes Human Pair 11 Lasers General Medicine medicine.disease Maxillary Diseases Cell Transformation Neoplastic Genetic marker Allelic Imbalance Odontogenic Cysts Chromosomes Human Pair 3 medicine.symptom Chromosomes Human Pair 9 Chromosomes Human Pair 17 |
| Zdroj: | Histopathology. 47(6) |
| ISSN: | 0309-0167 |
| Popis: | Aims: The bland histology of odontogenic keratocyst (OKC) belies its capacity for aggressive behaviour. Genetic alterations of OKC have not been well studied. We examined the frequency and pattern of allelic imbalance on five different chromosome regions from 15 patients with OKC. Methods and results: Laser-assisted microdissection was performed on formalin-fixed paraffin-embedded tissue. Polymerase chain reaction analysis of extracted DNA targeted five polymorphic DNA markers (D3S1285, D9S161, D11S1316, D13S290, and TP53) representing chromosome regions 3p14, 9p21, 11q23, 13q12.1 and 17p13, respectively. All 15 cases of OKC were informative at a minimum of three of five loci, with 11 informative on all five loci. Twelve of 15 cases (80%) demonstrated loss of heterozygosity (LOH). Seven cases (47%) showed LOH at more than two DNA loci. The frequency of LOH was 5 ⁄ 11 (45%) at D3S1285, 3 ⁄ 15 (20%) at D9S161, 4 ⁄ 14 (29%) at D11S1316, 8 ⁄ 14 (57%) at D13S290 and 3 ⁄ 15 (20%) at TP53. Conclusions: The majority of OKCs harbour chromosomal abnormalities. This finding supports the supposition that OKCs are neoplastic. Furthermore, OKCs harbour allelic loss at some of the same loci identified in squamous cell carcinoma. This may aid in explaining the rare occurrence of squamous cell carcinoma arising in OKC. |
| Databáze: | OpenAIRE |
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