Fecal Volatile Metabolomics Predict Gram-Negative Late-Onset Sepsis in Preterm Infants: A Nationwide Case-Control Study
| Autor: | Nina M. Frerichs, Sofia el Manouni el Hassani, Nancy Deianova, Mirjam M. van Weissenbruch, Anton H. van Kaam, Daniel C. Vijlbrief, Johannes B. van Goudoever, Christian V. Hulzebos, Boris. W. Kramer, Esther J. d’Haens, Veerle Cossey, Willem P. de Boode, Wouter J. de Jonge, Alfian N. Wicaksono, James A. Covington, Marc A. Benninga, Nanne K. H. de Boer, Hendrik J. Niemarkt, Tim G. J. de Meij |
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| Přispěvatelé: | RS: MHeNs - R3 - Neuroscience, RS: GROW - R4 - Reproductive and Perinatal Medicine, MUMC+: MA Medische Staf Kindergeneeskunde (9), Kindergeneeskunde, Pediatrics, Amsterdam Gastroenterology Endocrinology Metabolism, Amsterdam Reproduction & Development (AR&D), Gastroenterology and hepatology, Paediatric Gastroenterology, AGEM - Endocrinology, metabolism and nutrition, Graduate School, Tytgat Institute for Liver and Intestinal Research, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Neonatology, ARD - Amsterdam Reproduction and Development, Gastroenterology and Hepatology, AII - Inflammatory diseases |
| Rok vydání: | 2023 |
| Předmět: |
BIRTH-WEIGHT INFANTS
Microbiology (medical) gas chromatography-ion mobility spectrometry Vascular damage Radboud Institute for Health Sciences [Radboudumc 16] fecal biomarker gas chromatography-time of flight-mass spectrometry neonatology Microbiology DISEASE TRANSLOCATION MICROBIOME gas chromatography—ion mobility spectrometry volatile organic compounds Virology BACTERIA VOLUME microbiota GUT ORGANIC-COMPOUNDS GESTATIONAL-AGE gas chromatography—time of flight—mass spectrometry |
| Zdroj: | Microorganisms, 11, 3 Microorganisms, 11(3):572. Multidisciplinary Digital Publishing Institute (MDPI) Microorganisms, 11(3):572. MDPI AG Microorganisms Volume 11 Issue 3 Pages: 572 Microorganisms, 11 Frerichs, N M, El Manouni El Hassani, S, Deianova, N, van Weissenbruch, M M, van Kaam, A H, Vijlbrief, D C, van Goudoever, J B, Hulzebos, C V, Kramer, B W, d'Haens, E J, Cossey, V, de Boode, W P, de Jonge, W J, Wicaksono, A N, Covington, J A, Benninga, M A, de Boer, N K H, Niemarkt, H J & de Meij, T G J 2023, ' Fecal Volatile Metabolomics Predict Gram-Negative Late-Onset Sepsis in Preterm Infants : A Nationwide Case-Control Study ', Microorganisms, vol. 11, no. 3, 572 . https://doi.org/10.3390/microorganisms11030572 |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms11030572 |
| Popis: | Contains fulltext : 291609.pdf (Publisher’s version ) (Open Access) Early detection of late-onset sepsis (LOS) in preterm infants is crucial since timely treatment initiation is a key prognostic factor. We hypothesized that fecal volatile organic compounds (VOCs), reflecting microbiota composition and function, could serve as a non-invasive biomarker for preclinical pathogen-specific LOS detection. Fecal samples and clinical data of all preterm infants (≤30 weeks' gestation) admitted at nine neonatal intensive care units in the Netherlands and Belgium were collected daily. Samples from one to three days before LOS onset were analyzed by gas chromatography-ion mobility spectrometry (GC-IMS), a technique based on pattern recognition, and gas chromatography-time of flight-mass spectrometry (GC-TOF-MS), to identify unique metabolites. Fecal VOC profiles and metabolites from infants with LOS were compared with matched controls. Samples from 121 LOS infants and 121 matched controls were analyzed using GC-IMS, and from 34 LOS infants and 34 matched controls using GC-TOF-MS. Differences in fecal VOCs were most profound one and two days preceding Escherichia coli LOS (Area Under Curve; p-value: 0.73; p = 0.02, 0.83; p < 0.002, respectively) and two and three days before gram-negative LOS (0.81; p < 0.001, 0.85; p < 0.001, respectively). GC-TOF-MS identified pathogen-specific discriminative metabolites for LOS. This study underlines the potential for VOCs as a non-invasive preclinical diagnostic LOS biomarker. |
| Databáze: | OpenAIRE |
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