Rice OsAAA-ATPase1 is Induced during Blast Infection in a Salicylic Acid-Dependent Manner, and Promotes Blast Fungus Resistance
Autor: | Xianying Tang, Yanping Tan, Chuntai Wang, Xinqiong Liu, Haruhiko Inoue, Xin Xu, Chang-Jie Jiang |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine disease resistance Xanthomonas salicylic acid Plant disease resistance medicine.disease_cause 01 natural sciences Catalysis Article Microbiology Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Xanthomonas oryzae Gene expression Plant defense against herbivory medicine Physical and Theoretical Chemistry Molecular Biology Gene Escherichia coli lcsh:QH301-705.5 AAA-ATPase Spectroscopy Plant Diseases Plant Proteins Adenosine Triphosphatases biology rice Organic Chemistry fungi food and beverages Oryza General Medicine Magnaporthe oryzae biology.organism_classification Genetically modified rice Computer Science Applications Magnaporthe 030104 developmental biology chemistry lcsh:Biology (General) lcsh:QD1-999 fatty acid magnaporthe oryzae Salicylic acid 010606 plant biology & botany |
Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 4, p 1443 (2020) Volume 21 Issue 4 |
ISSN: | 1422-0067 |
Popis: | Fatty acids (FAs) have been implicated in signaling roles in plant defense responses. We previously reported that mutation or RNAi-knockdown (OsSSI2-kd) of the rice OsSSI2 gene, encoding a stearoyl acyl carrier protein FA desaturase (SACPD), remarkably enhanced resistance to blast fungus Magnaporthe oryzae and the leaf-blight bacterium Xanthomonas oryzae pv. oryzae (Xoo). Transcriptomic analysis identified six AAA-ATPase family genes (hereafter OsAAA-ATPase1&ndash 6) upregulated in the OsSSI2-kd plants, in addition to other well-known defense-related genes. Here, we report the functional analysis of OsAAA-ATPase1 in rice&rsquo s defense response to M. oryzae. Recombinant OsAAA-ATPase1 synthesized in Escherichia coli showed ATPase activity. OsAAA-ATPase1 transcription was induced by exogenous treatment with a functional analogue of salicylic acid (SA), benzothiadiazole (BTH), but not by other plant hormones tested. The transcription of OsAAA-ATPase1 was also highly induced in response to M. oryzae infection in an SA-dependent manner, as gene induction was significantly attenuated in a transgenic rice line expressing a bacterial gene (nahG) encoding salicylate hydroxylase. Overexpression of OsAAA-ATPase1 significantly enhanced pathogenesis-related gene expression and the resistance to M. oryzae conversely, RNAi-mediated suppression of this gene compromised this resistance. These results suggest that OsAAA-APTase1 plays an important role in SA-mediated defense responses against blast fungus M. oryzae. |
Databáze: | OpenAIRE |
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