Rice OsAAA-ATPase1 is Induced during Blast Infection in a Salicylic Acid-Dependent Manner, and Promotes Blast Fungus Resistance

Autor: Xianying Tang, Yanping Tan, Chuntai Wang, Xinqiong Liu, Haruhiko Inoue, Xin Xu, Chang-Jie Jiang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
disease resistance
Xanthomonas
salicylic acid
Plant disease resistance
medicine.disease_cause
01 natural sciences
Catalysis
Article
Microbiology
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
chemistry.chemical_compound
Xanthomonas oryzae
Gene expression
Plant defense against herbivory
medicine
Physical and Theoretical Chemistry
Molecular Biology
Gene
Escherichia coli
lcsh:QH301-705.5
AAA-ATPase
Spectroscopy
Plant Diseases
Plant Proteins
Adenosine Triphosphatases
biology
rice
Organic Chemistry
fungi
food and beverages
Oryza
General Medicine
Magnaporthe oryzae
biology.organism_classification
Genetically modified rice
Computer Science Applications
Magnaporthe
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
fatty acid
magnaporthe oryzae
Salicylic acid
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 21, Iss 4, p 1443 (2020)
Volume 21
Issue 4
ISSN: 1422-0067
Popis: Fatty acids (FAs) have been implicated in signaling roles in plant defense responses. We previously reported that mutation or RNAi-knockdown (OsSSI2-kd) of the rice OsSSI2 gene, encoding a stearoyl acyl carrier protein FA desaturase (SACPD), remarkably enhanced resistance to blast fungus Magnaporthe oryzae and the leaf-blight bacterium Xanthomonas oryzae pv. oryzae (Xoo). Transcriptomic analysis identified six AAA-ATPase family genes (hereafter OsAAA-ATPase1&ndash
6) upregulated in the OsSSI2-kd plants, in addition to other well-known defense-related genes. Here, we report the functional analysis of OsAAA-ATPase1 in rice&rsquo
s defense response to M. oryzae. Recombinant OsAAA-ATPase1 synthesized in Escherichia coli showed ATPase activity. OsAAA-ATPase1 transcription was induced by exogenous treatment with a functional analogue of salicylic acid (SA), benzothiadiazole (BTH), but not by other plant hormones tested. The transcription of OsAAA-ATPase1 was also highly induced in response to M. oryzae infection in an SA-dependent manner, as gene induction was significantly attenuated in a transgenic rice line expressing a bacterial gene (nahG) encoding salicylate hydroxylase. Overexpression of OsAAA-ATPase1 significantly enhanced pathogenesis-related gene expression and the resistance to M. oryzae
conversely, RNAi-mediated suppression of this gene compromised this resistance. These results suggest that OsAAA-APTase1 plays an important role in SA-mediated defense responses against blast fungus M. oryzae.
Databáze: OpenAIRE
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