| Popis: |
PDF file, 6751K, Figure S2. (A) SK1 mRNA expression in MCF7 cells treated with 10nM of estradiol (E2). MCF7 were treated with 10nM E2 or ethanol (negative control). After 3, 6, 12, 24 and 48 hours of treatment, SK1 mRNA levels were measured by RT-qPCR. For each time point, SK1 mRNA levels represent the ratio between the values obtained for E2 and ethanol. (B) miR-515-5p expression in ZR75-1 treated with 10nM of estradiol (E2). MCF7 were treated with 10nM E2 or ethanol (negative control). After 3, 6, 12, 24 and 48 hours of treatment, miR 515-5p levels were measured by RT-qPCR. For each time point, miR 515-5p levels represent the ratio between the values obtained for E2 and ethanol. (C) SK1 mRNA levels and phospho-ERK 1/2 protein expression in MCF7 cells treated with TAM. MCF7 cells were treated with 100nM TAM and ethanol. After 48 hours, SK1 mRNA levels were measured by RT-qPCR and (D) phospho-ERK 1/2 protein levels were analyzed by immuno-blotting. Data presented are the mean plus-minus SEM (*P |
