Characterization of a novel class of glyoxylate reductase belonging to the β-hydroxyacid dehydrogenase family in Acetobacter aceti
| Autor: | Jakkaphan Kumsab, Hisaaki Mihara, Ryuta Tobe, Taketo Omori, Tatsuo Kurihara, Yuu Hirose |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Hydroxypyruvate reductase Glyoxylate cycle Dehydrogenase Applied Microbiology and Biotechnology Biochemistry Cofactor Analytical Chemistry Succinic semialdehyde 03 medical and health sciences chemistry.chemical_compound Molecular Biology Glyoxylate reductase Acetobacter aceti chemistry.chemical_classification 030102 biochemistry & molecular biology biology Organic Chemistry General Medicine biology.organism_classification 030104 developmental biology Enzyme chemistry biology.protein Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 84:2303-2310 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2020.1797470 |
| Popis: | Enzymes related to β-hydroxyacid dehydrogenases/3-hydroxyisobutyrate dehydrogenases are ubiquitous, but most of them have not been characterized. An uncharacterized protein with moderate sequence similarities to Gluconobacter oxydans succinic semialdehyde reductase and plant glyoxylate reductases/succinic semialdehyde reductases was found in the genome of Acetobacter aceti JCM20276. The corresponding gene was cloned and expressed in Escherichia coli. The gene product was purified and identified as a glyoxylate reductase that exclusively catalyzed the NAD(P)H-dependent reduction of glyoxylate to glycolate. The strict substrate specificity of this enzyme to glyoxylate, the diverged sequence motifs for its binding sites with cofactors and substrates, and its phylogenetic relationship to homologous enzymes suggested that this enzyme represents a novel class of enzymes in the β-hydroxyacid dehydrogenase family. This study may provide an important clue to clarify the metabolism of glyoxylate in bacteria. Abbreviations: GR: glyoxylate reductase; GRHPR: glyoxylate reductase/hydroxypyruvate reductase; HIBADH: 3-hydroxyisobutyrate dehydrogenase; SSA: succinic semialdehyde; SSAR: succinic semialdehyde reductase AceGR is NAD(P)H-dependent glyoxylate reductase representing a novel class of enzymes in the β-hydroxyacid dehydrogenase family. |
| Databáze: | OpenAIRE |
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